The chromodomain protein, chromator, interacts with JIL-1 kinase and regulates the structure of Drosophila polytene chromosomes

Uttama Rath, Yun Ding, Huai Deng, Hongying Qi, Xiaomin Bao, Weiguo Zhang, Jack Girton, Jørgen Johansen, Kristen M. Johansen*

*Corresponding author for this work

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

In this study we have generated two new hypomorphic Chro alleles and analyzed the consequences of reduced Chromator protein function on polytene chromosome structure. We show that in Chro71/Chro612 mutants the polytene chromosome arms were coiled and compacted with a disruption and misalignment of band and interband regions and with numerous ectopic contacts connecting non-homologous regions. Furthermore, we demonstrate that Chromator co-localizes with the JIL-1 kinase at polytene interband regions and that the two proteins interact within the same protein complex. That both proteins are necessary and may function together is supported by the finding that a concomitant reduction in JIL-1 and Chromator function synergistically reduces viability during development Overlay assays and deletion construct analysis suggested that the interaction between JIL-1 and Chromator is direct and that it is mediated by sequences in the C-terminal domain of Chromator and by the acidic region within the C-terminal domain of JIL-1. Taken together these findings indicate that Chromator and JIL-1 interact in an interband-specific complex that functions to establish or maintain polytene chromosome structure in Drosophila.

Original languageEnglish (US)
Pages (from-to)2332-2341
Number of pages10
JournalJournal of cell science
Volume119
Issue number11
DOIs
StatePublished - Jun 1 2006

Keywords

  • Chromatin structure
  • Chromator
  • Chromodomain
  • Drosophila
  • JIL-1 kinase

ASJC Scopus subject areas

  • Cell Biology

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