The composition and structure of bovine peritubular dentin: Mapping by time of flight secondary ion mass spectroscopy

Bat Ami Gotliv, Joshua S. Robach, Arthur Veis*

*Corresponding author for this work

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

The dentin layer of the tooth is a complex mineralized tissue traversed by a closely packed system of tubules. Each tubule is surrounded by highly mineralized tissue referred to as peritubular dentin (PTD). The remaining mineralized collagen network between the tubules is the intertubular dentin (ITD). A TOF-SIMS analysis of the PTD constituents has been used to compare the PTD to the ITD. The PTD differs from the ITD not only in the degree of mineralization but also in the amount and nature of the mineral elements and amino acids. The organic matrix of the PTD consists of a unique collagen free assembly of proteins rich in glutamic acid, where the ITD organic matrix is collagen-rich and Asp-rich. The apparent concentration of organic fragment ions observed in the PTD in the TOF-SIMS negative ion mode was much higher than expected. The PTD was found to be rich in Ca2+, Na+, Mg2+, and K+. The amount of Mg2+ and K+ in the PTD was significantly reduced after deproteination, while Ca2+ and Na+ were still accumulated in the PTD. This implies that Mg2+ and K+ are mainly associated with the organic matrix rather than with the mineral of the PTD.

Original languageEnglish (US)
Pages (from-to)320-333
Number of pages14
JournalJournal of Structural Biology
Volume156
Issue number2
DOIs
StatePublished - Nov 1 2006

Keywords

  • Collagen
  • Intertubular dentin
  • Non-collagenous proteins
  • Peritubular dentin
  • TOF-SIMS
  • Tooth structure

ASJC Scopus subject areas

  • Structural Biology

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