The concomitant augmentation of urokinase-type plasminogen activator and collagenase-like proteinase activities in X-ray irradiated cells of a human metastatic carcinomatous line

Nobue Uchida, Kei Takahashi, Toshifumi Kasai, Tetsuya Ishida, Hau C. Kwaan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The enzymatic activities of uPA, and a collagenase-like proteinase in the post-nuclear fraction of cell homogenates of a metastatic carcinomatous cell line following X-ray irradiation were examined by the use of chromogenic substrates and by casein- or gelatin-containing zymographies and electrophoretic gel stained with avidin-conjugated peroxidase. Enhanced activities were observed in these cells, while those of 5′nucleotidase and Na+-K+-ATPase were attenuated. A partial purification and characterization of the collagenase showed that it was able to hydrolyze the heat-denatured type-I collagen more efficiently than the native one. The activation of both uPA and collagenase enables an efficient degradation of matrix barrier proteins. These findings suggest that following a certain dose range of X-ray irradiation, tumor cells may increase their ability to migrate and invade through the enhancement of uPA and collagenase activities.

Original languageEnglish (US)
Pages (from-to)565-576
Number of pages12
JournalThrombosis research
Volume74
Issue number6
DOIs
StatePublished - Jun 15 1994

Keywords

  • Urokinase
  • collagenase
  • receptor
  • type-I collagen

ASJC Scopus subject areas

  • Hematology

Fingerprint Dive into the research topics of 'The concomitant augmentation of urokinase-type plasminogen activator and collagenase-like proteinase activities in X-ray irradiated cells of a human metastatic carcinomatous line'. Together they form a unique fingerprint.

Cite this