The condensin complex is required for proper spindle assembly and chromosome segregation in Xenopus egg extracts

Sarah M. Wignall, Renée Deehan, Thomas J. Maresca, Rebecca Heald*

*Corresponding author for this work

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Chromosome condensation is required for the physical resolution and segregation of sister chromatids during cell division, but the precise role of higher order chromatin structure in mitotic chromosome functions is unclear. Here, we address the role of the major condensation machinery, the condensin complex, in spindle assembly and function in Xenopus laevis egg extracts. Immunodepletion of condensin inhibited microtubule growth and organization around chromosomes, reducing the percentage of sperm nuclei capable of forming spindles, and causing dramatic defects in anaphase chromosome segregation. Although the motor CENP-E was recruited to kinetochores pulled poleward during anaphase, the disorganized chromosome mass was not resolved. Inhibition of condensin function during anaphase also inhibited chromosome segregation, indicating its continuous requirement. Spindle assembly around DNA-coated beads in the absence of kinetochores was also impaired upon condensin inhibition. These results support an important role for condensin in establishing chromosomal architecture necessary for proper spindle assembly and chromosome segregation.

Original languageEnglish (US)
Pages (from-to)1041-1051
Number of pages11
JournalJournal of Cell Biology
Volume161
Issue number6
DOIs
StatePublished - Jun 23 2003

Keywords

  • Anaphase
  • Condensation
  • Kinetochore
  • Microtubule
  • Mitosis

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'The condensin complex is required for proper spindle assembly and chromosome segregation in Xenopus egg extracts'. Together they form a unique fingerprint.

  • Cite this