The CpG‐rich promoter of human LDH‐C is differentially methylated in expressing and nonexpressing tissues

Christophe Bonny, Erwin Goldberg*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

20 Scopus citations

Abstract

A comparison of nucleolide sequences of murine LDH‐a and Ldh‐c genes and human LDH‐A, LDH‐B, and LDH‐C reveals that mouse Ldh‐c has lost the CpG “island” present in the genes for the somatic isozymes. However, the human LDH‐C gene has a CpG‐rich region of 230 bp surrounding its promoter. Endonuclease sensitivity coupled with polymerase chain reaction (PCR) demonstrate the presence of nine heavily methylated sites in this region in different somatic cells. The same sites are specifically hypomethy‐lated in expressing tissues. 3′ sites bordering the CpG‐rich region appear to be methylated in both expressing and nonexpressing tissues. Furthermore, the methylated promoter forms a specific complex in vitro with a methyl‐DNA binding protein. Evolutionary and functional implications of these observations are discussed. © 1995 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)210-217
Number of pages8
JournalDevelopmental Genetics
Volume16
Issue number2
DOIs
StatePublished - 1995

Keywords

  • CpG island
  • gene expression
  • lactate dehydrogenase C
  • methylation
  • promoter
  • testis

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

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