The CRISPR tool kit for genome editing and beyond

Mazhar Adli

doi:10.1038/s41467-018-04252-2

The CRISPR tool kit for genome editing and beyond

Mazhar Adli^*

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

348 Scopus citations

Abstract

CRISPR is becoming an indispensable tool in biological research. Once known as the bacterial immune system against invading viruses, the programmable capacity of the Cas9 enzyme is now revolutionizing diverse fields of medical research, biotechnology, and agriculture. CRISPR-Cas9 is no longer just a gene-editing tool; the application areas of catalytically impaired inactive Cas9, including gene regulation, epigenetic editing, chromatin engineering, and imaging, now exceed the gene-editing functionality of WT Cas9. Here, we will present a brief history of gene-editing tools and describe the wide range of CRISPR-based genome-targeting tools. We will conclude with future directions and the broader impact of CRISPR technologies.

Original language	English (US)
Article number	1911
Journal	Nature communications
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41467-018-04252-2
State	Published - Dec 1 2018
Externally published	Yes

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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10.1038/s41467-018-04252-2

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title = "The CRISPR tool kit for genome editing and beyond",

abstract = "CRISPR is becoming an indispensable tool in biological research. Once known as the bacterial immune system against invading viruses, the programmable capacity of the Cas9 enzyme is now revolutionizing diverse fields of medical research, biotechnology, and agriculture. CRISPR-Cas9 is no longer just a gene-editing tool; the application areas of catalytically impaired inactive Cas9, including gene regulation, epigenetic editing, chromatin engineering, and imaging, now exceed the gene-editing functionality of WT Cas9. Here, we will present a brief history of gene-editing tools and describe the wide range of CRISPR-based genome-targeting tools. We will conclude with future directions and the broader impact of CRISPR technologies.",

author = "Mazhar Adli",

note = "Funding Information: This work is supported by grants from National Institute of Health (NIH; 1RO1 CA211648-01) and National Science Foundation (NSF; MCB 1715183). Funding Information: I would like to thank all of the members of the Adli lab for their critical readings and thoughtful recommendations. This work is supported by grants from National Institute of Health (NIH; 1RO1 CA211648-01) and National Science Foundation (NSF; MCB 1715183). While writing this review, I have attempted to cite the original research articles as much as possible. However, given the space limitations and the exponential growth of the CRISPR field, I apologize if I unintentionally missed citing some of the original work that deserved citation.",

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