The DEK oncoprotein is a Su(var) that is essential to heterochromatin integrity

Ferdinand Kappes; Tanja Waldmann; Veena Mathew; Jindan Yu; Ling Zhang; Michael S. Khodadoust; Arul M. Chinnaiyan; Karolin Luger; Sylvia Erhardt; Robert Schneider; David M. Markovitz

doi:10.1101/gad.2036411

The DEK oncoprotein is a Su(var) that is essential to heterochromatin integrity

Ferdinand Kappes, Tanja Waldmann, Veena Mathew, Jindan Yu, Ling Zhang, Michael S. Khodadoust, Arul M. Chinnaiyan, Karolin Luger, Sylvia Erhardt, Robert Schneider, David M. Markovitz

Medicine, Hematology Oncology Division

Research output: Contribution to journal › Article › peer-review

67 Scopus citations

Abstract

Heterochromatin integrity is crucial for genome stability and regulation of gene expression, but the factors involved in mammalian heterochromatin biology are only incompletely understood. Here we identify the oncoprotein DEK, an abundant nuclear protein with a previously enigmatic in vivo function, as a Suppressor of Variegation [Su(var)] that is crucial to global heterochromatin integrity. We show that DEK interacts directly with Heterochromatin Protein 1 a (HP1a) and markedly enhances its binding to trimethylated H3K9 (H3K9me3), which is key for maintaining heterochromatic regions. Loss of Dek in Drosophila leads to a Su(var) phenotype and global reduction in heterochromatin. Thus, these findings show that DEK is a key factor in maintaining the balance between heterochromatin and euchromatin in vivo.

Original language	English (US)
Pages (from-to)	673-678
Number of pages	6
Journal	Genes and Development
Volume	25
Issue number	7
DOIs	https://doi.org/10.1101/gad.2036411
State	Published - Apr 2011

Keywords

Epigenetics
HP1-α
Heterochromatin
Oncogene
Su(var)

ASJC Scopus subject areas

Genetics
Developmental Biology

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title = "The DEK oncoprotein is a Su(var) that is essential to heterochromatin integrity",

abstract = "Heterochromatin integrity is crucial for genome stability and regulation of gene expression, but the factors involved in mammalian heterochromatin biology are only incompletely understood. Here we identify the oncoprotein DEK, an abundant nuclear protein with a previously enigmatic in vivo function, as a Suppressor of Variegation [Su(var)] that is crucial to global heterochromatin integrity. We show that DEK interacts directly with Heterochromatin Protein 1 a (HP1a) and markedly enhances its binding to trimethylated H3K9 (H3K9me3), which is key for maintaining heterochromatic regions. Loss of Dek in Drosophila leads to a Su(var) phenotype and global reduction in heterochromatin. Thus, these findings show that DEK is a key factor in maintaining the balance between heterochromatin and euchromatin in vivo.",

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author = "Ferdinand Kappes and Tanja Waldmann and Veena Mathew and Jindan Yu and Ling Zhang and Khodadoust, {Michael S.} and Chinnaiyan, {Arul M.} and Karolin Luger and Sylvia Erhardt and Robert Schneider and Markovitz, {David M.}",

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AU - Kappes, Ferdinand

AU - Waldmann, Tanja

AU - Mathew, Veena

AU - Yu, Jindan

AU - Zhang, Ling

AU - Khodadoust, Michael S.

AU - Chinnaiyan, Arul M.

AU - Luger, Karolin

AU - Erhardt, Sylvia

AU - Schneider, Robert

AU - Markovitz, David M.

PY - 2011/4

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N2 - Heterochromatin integrity is crucial for genome stability and regulation of gene expression, but the factors involved in mammalian heterochromatin biology are only incompletely understood. Here we identify the oncoprotein DEK, an abundant nuclear protein with a previously enigmatic in vivo function, as a Suppressor of Variegation [Su(var)] that is crucial to global heterochromatin integrity. We show that DEK interacts directly with Heterochromatin Protein 1 a (HP1a) and markedly enhances its binding to trimethylated H3K9 (H3K9me3), which is key for maintaining heterochromatic regions. Loss of Dek in Drosophila leads to a Su(var) phenotype and global reduction in heterochromatin. Thus, these findings show that DEK is a key factor in maintaining the balance between heterochromatin and euchromatin in vivo.

AB - Heterochromatin integrity is crucial for genome stability and regulation of gene expression, but the factors involved in mammalian heterochromatin biology are only incompletely understood. Here we identify the oncoprotein DEK, an abundant nuclear protein with a previously enigmatic in vivo function, as a Suppressor of Variegation [Su(var)] that is crucial to global heterochromatin integrity. We show that DEK interacts directly with Heterochromatin Protein 1 a (HP1a) and markedly enhances its binding to trimethylated H3K9 (H3K9me3), which is key for maintaining heterochromatic regions. Loss of Dek in Drosophila leads to a Su(var) phenotype and global reduction in heterochromatin. Thus, these findings show that DEK is a key factor in maintaining the balance between heterochromatin and euchromatin in vivo.

KW - Epigenetics

KW - HP1-α

KW - Heterochromatin

KW - Oncogene

KW - Su(var)

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JO - Genes and Development

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IS - 7

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The DEK oncoprotein is a Su(var) that is essential to heterochromatin integrity

Abstract

Keywords

ASJC Scopus subject areas

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