The differential regulation of steroidogenic acute regulatory protein-mediated steroidogenesis by type I and type II PKA in MA-10 cells

Matthew T. Dyson, Mariusz P. Kowalewski, Pulak R. Manna, Douglas M. Stocco*

*Corresponding author for this work

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

Following tropic hormone challenge, steroidogenic tissues utilize PKA to phosphorylate unique subsets of proteins necessary to facilitate steroidogenesis. This includes the PKA-dependent expression and activation of the steroidogenic acute regulatory protein (STAR), which mediates the rate-limiting step of steroidogenesis by inducing the transfer of cholesterol from the outer to the inner mitochondrial membrane. Since both type I and type II PKA are present in steroidogenic tissues, we have utilized cAMP analog pairs that preferentially activate each PKA subtype in order to examine their impact on STAR synthesis and activity. In MA-10 mouse Leydig tumor cells Star gene expression is more dependent upon type I PKA, while the post-transcriptional regulation of STAR appears subject to type II PKA. These experiments delineate the discrete effects that type I and type II PKA exert on STAR-mediated steroidogenesis, and suggest complimentary roles for each subtype in coordinating steroidogenesis.

Original languageEnglish (US)
Pages (from-to)94-103
Number of pages10
JournalMolecular and Cellular Endocrinology
Volume300
Issue number1-2
DOIs
StatePublished - Mar 5 2009

Keywords

  • Leydig
  • PKA
  • STAR
  • Steroidogenesis
  • cAMP
  • cAMP-dependent protein kinase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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