The differential regulation of steroidogenic acute regulatory protein-mediated steroidogenesis by type I and type II PKA in MA-10 cells

Matthew T. Dyson; Mariusz P. Kowalewski; Pulak R. Manna; Douglas M. Stocco

doi:10.1016/j.mce.2008.11.029

The differential regulation of steroidogenic acute regulatory protein-mediated steroidogenesis by type I and type II PKA in MA-10 cells

Matthew T. Dyson, Mariusz P. Kowalewski, Pulak R. Manna, Douglas M. Stocco^*

^*Corresponding author for this work

Obstetrics and Gynecology

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

Following tropic hormone challenge, steroidogenic tissues utilize PKA to phosphorylate unique subsets of proteins necessary to facilitate steroidogenesis. This includes the PKA-dependent expression and activation of the steroidogenic acute regulatory protein (STAR), which mediates the rate-limiting step of steroidogenesis by inducing the transfer of cholesterol from the outer to the inner mitochondrial membrane. Since both type I and type II PKA are present in steroidogenic tissues, we have utilized cAMP analog pairs that preferentially activate each PKA subtype in order to examine their impact on STAR synthesis and activity. In MA-10 mouse Leydig tumor cells Star gene expression is more dependent upon type I PKA, while the post-transcriptional regulation of STAR appears subject to type II PKA. These experiments delineate the discrete effects that type I and type II PKA exert on STAR-mediated steroidogenesis, and suggest complimentary roles for each subtype in coordinating steroidogenesis.

Original language	English (US)
Pages (from-to)	94-103
Number of pages	10
Journal	Molecular and Cellular Endocrinology
Volume	300
Issue number	1-2
DOIs	https://doi.org/10.1016/j.mce.2008.11.029
State	Published - Mar 5 2009

Funding

We would like to acknowledge Yuping Sun for her technical assistance. This research was supported by funds from the NIH grant # HD-17481 and grant # B1-0028 from the Robert A. Welch Foundation (to D.M.S.).

Keywords

Leydig
PKA
STAR
Steroidogenesis
cAMP
cAMP-dependent protein kinase

ASJC Scopus subject areas

Endocrinology
Molecular Biology
Biochemistry

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10.1016/j.mce.2008.11.029

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title = "The differential regulation of steroidogenic acute regulatory protein-mediated steroidogenesis by type I and type II PKA in MA-10 cells",

abstract = "Following tropic hormone challenge, steroidogenic tissues utilize PKA to phosphorylate unique subsets of proteins necessary to facilitate steroidogenesis. This includes the PKA-dependent expression and activation of the steroidogenic acute regulatory protein (STAR), which mediates the rate-limiting step of steroidogenesis by inducing the transfer of cholesterol from the outer to the inner mitochondrial membrane. Since both type I and type II PKA are present in steroidogenic tissues, we have utilized cAMP analog pairs that preferentially activate each PKA subtype in order to examine their impact on STAR synthesis and activity. In MA-10 mouse Leydig tumor cells Star gene expression is more dependent upon type I PKA, while the post-transcriptional regulation of STAR appears subject to type II PKA. These experiments delineate the discrete effects that type I and type II PKA exert on STAR-mediated steroidogenesis, and suggest complimentary roles for each subtype in coordinating steroidogenesis.",

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author = "Dyson, {Matthew T.} and Kowalewski, {Mariusz P.} and Manna, {Pulak R.} and Stocco, {Douglas M.}",

note = "Funding Information: We would like to acknowledge Yuping Sun for her technical assistance. This research was supported by funds from the NIH grant # HD-17481 and grant # B1-0028 from the Robert A. Welch Foundation (to D.M.S.).",

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AU - Manna, Pulak R.

AU - Stocco, Douglas M.

N1 - Funding Information: We would like to acknowledge Yuping Sun for her technical assistance. This research was supported by funds from the NIH grant # HD-17481 and grant # B1-0028 from the Robert A. Welch Foundation (to D.M.S.).

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N2 - Following tropic hormone challenge, steroidogenic tissues utilize PKA to phosphorylate unique subsets of proteins necessary to facilitate steroidogenesis. This includes the PKA-dependent expression and activation of the steroidogenic acute regulatory protein (STAR), which mediates the rate-limiting step of steroidogenesis by inducing the transfer of cholesterol from the outer to the inner mitochondrial membrane. Since both type I and type II PKA are present in steroidogenic tissues, we have utilized cAMP analog pairs that preferentially activate each PKA subtype in order to examine their impact on STAR synthesis and activity. In MA-10 mouse Leydig tumor cells Star gene expression is more dependent upon type I PKA, while the post-transcriptional regulation of STAR appears subject to type II PKA. These experiments delineate the discrete effects that type I and type II PKA exert on STAR-mediated steroidogenesis, and suggest complimentary roles for each subtype in coordinating steroidogenesis.

AB - Following tropic hormone challenge, steroidogenic tissues utilize PKA to phosphorylate unique subsets of proteins necessary to facilitate steroidogenesis. This includes the PKA-dependent expression and activation of the steroidogenic acute regulatory protein (STAR), which mediates the rate-limiting step of steroidogenesis by inducing the transfer of cholesterol from the outer to the inner mitochondrial membrane. Since both type I and type II PKA are present in steroidogenic tissues, we have utilized cAMP analog pairs that preferentially activate each PKA subtype in order to examine their impact on STAR synthesis and activity. In MA-10 mouse Leydig tumor cells Star gene expression is more dependent upon type I PKA, while the post-transcriptional regulation of STAR appears subject to type II PKA. These experiments delineate the discrete effects that type I and type II PKA exert on STAR-mediated steroidogenesis, and suggest complimentary roles for each subtype in coordinating steroidogenesis.

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KW - cAMP

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The differential regulation of steroidogenic acute regulatory protein-mediated steroidogenesis by type I and type II PKA in MA-10 cells

Abstract

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Keywords

ASJC Scopus subject areas

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