The Drosophila centrosomal protein Nuf is required for recruiting Dah, a membrane associated protein, to furrows in the early embryo

Wendy F. Rothwell, Claire X. Zhang, Christina Zelano, Tao Shih Hsieh, William Sullivan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

During mitosis of the Drosophila cortical syncytial divisions, actin-based membrane furrows separate adjacent spindles. Our genetic analysis indicates that the centrosomal protein Nuf is specifically required for recruitment of components to the furrows and the membrane-associated protein Dah is primarily required for the inward invagination of the furrow membrane. Recruitment of actin, anillin and peanut to the furrows occurs normally in dah-derived embryos. However, subsequent invagination of the furrows fails in dah-derived embryos and the septins become dispersed throughout the cytoplasm. This indicates that stable septin localization requires Dah-mediated furrow invagination. Close examination of actin and Dah localization in wild-type embryos reveals that they associate in adjacent particles during interphase and co-localize in the invaginating furrows during prophase and metaphase. We show that the Nuf centrosomal protein is required for recruiting the membrane-associated protein Dah to the furrows. In nuf-mutant embryos, much of the Dah does not reach the furrows and remains in a punctate distribution. This suggests that Dah is recruited to the furrows in vesicles and that the recruiting step is disrupted in nuf mutants. These studies lead to a model in which the centrosomes play an important role in the transport of membrane-associated proteins and other components to the developing furrows.

Original languageEnglish (US)
Pages (from-to)2885-2893
Number of pages9
JournalJournal of cell science
Volume112
Issue number17
StatePublished - 1999

Keywords

  • Actin
  • Centrosome
  • Drosophila
  • Embryo
  • Membrane
  • Septin

ASJC Scopus subject areas

  • Cell Biology

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