The dynamic properties of intermediate filaments during organelle transport

Lynne Chang, Kari Barlan, Ying Hao Chou, Boris Grin, Margot Lakonishok, Anna S. Serpinskaya, Dale K. Shumaker, Harald Herrmann, Vladimir I. Gelfand*, Robert D. Goldman

*Corresponding author for this work

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

Intermediate filament (IF) dynamics during organelle transport and their role in organelle movement were studied using Xenopus laevis melanophores. In these cells, pigment granules (melanosomes) move along microtubules and microfilaments, toward and away from the cell periphery in response to α-melanocyte stimulating hormone (α-MSH) and melatonin, respectively. In this study we show that melanophores possess a complex network of vimentin IFs which interact with melanosomes. IFs form an intricate, honeycomb-like network that form cages surrounding individual and small clusters of melanosomes, both when they are aggregated and dispersed. Purified melanosome preparations contain a substantial amount of vimentin, suggesting that melanosomes bind to IFs. Analyses of individual melanosome movements in cells with disrupted IF networks show increased movement of granules in both anterograde and retrograde directions, further supporting the notion of a melanosome-IF interaction. Live imaging reveals that IFs, in turn, become highly flexible as melanosomes disperse in response to α-MSH. During the height of dispersion there is a marked increase in the rate of fluorescence recovery after photobleaching of GFP-vimentin IFs and an increase in vimentin solubility. These results reveal a dynamic interaction between membrane bound pigment granules and IFs and suggest a role for IFs as modulators of granule movement.

Original languageEnglish (US)
Pages (from-to)2914-2923
Number of pages10
JournalJournal of cell science
Volume122
Issue number16
DOIs
StatePublished - Aug 15 2009

Keywords

  • Intermediate filament
  • Melanosome
  • Transport
  • Vimentin

ASJC Scopus subject areas

  • Cell Biology

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    Chang, L., Barlan, K., Chou, Y. H., Grin, B., Lakonishok, M., Serpinskaya, A. S., Shumaker, D. K., Herrmann, H., Gelfand, V. I., & Goldman, R. D. (2009). The dynamic properties of intermediate filaments during organelle transport. Journal of cell science, 122(16), 2914-2923. https://doi.org/10.1242/jcs.046789