The effect of complete carcinogens on intercellular transfer of lucifer yellow in fibroblast culture

Irene V. Budunova; Leonid A. Mittelman; Gennady A. Belitsky

doi:10.1007/BF00135026

The effect of complete carcinogens on intercellular transfer of lucifer yellow in fibroblast culture

Irene V. Budunova^*, Leonid A. Mittelman, Gennady A. Belitsky

^*Corresponding author for this work

Dermatology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The effect on permeability of gap junctions of complete powerful carcinogens, 3-methylcholanthrene (MC), 7, 12-dimethylbenz(a)anthracene (DMBA), ethyl methanesulfonate (EMS), and weak carcinogens, benz(a)anthracene (BA), benzo(e)pyrene (B(e)P) as well as the arylhydrolase inhibitor 7,8-benzoflavone (7,8-BF) has been studied with the use of a dye-coupling technique and transformed Djungarian hamster DM15 fibroblasts. MC, EMS and 7,8-BF were found to exert a strong inhibitory effect on cell-to-cell dye transfer. BA and DMBA had the uncoupling activity only in 2 out of 4 experiments. B(e)P was not shown to affect LY transfer between DM15 cells. The uncoupling effect of MC, 7,8-BF and EMS (only when EMS used at the concentration of 600 μg/ ml but not 1000 μ/ ml) appeared reversible. The causes of failure to detect DMBA and B(e)P effects on gap junctions are discussed.

Original language	English (US)
Pages (from-to)	47-61
Number of pages	15
Journal	Cell Biology and Toxicology
Volume	6
Issue number	1
DOIs	https://doi.org/10.1007/BF00135026
State	Published - Jan 1990

Keywords

complete carcinogens
dye transfer
intercellular communication

ASJC Scopus subject areas

Toxicology
Cell Biology
Health, Toxicology and Mutagenesis

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10.1007/BF00135026

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title = "The effect of complete carcinogens on intercellular transfer of lucifer yellow in fibroblast culture",

abstract = "The effect on permeability of gap junctions of complete powerful carcinogens, 3-methylcholanthrene (MC), 7, 12-dimethylbenz(a)anthracene (DMBA), ethyl methanesulfonate (EMS), and weak carcinogens, benz(a)anthracene (BA), benzo(e)pyrene (B(e)P) as well as the arylhydrolase inhibitor 7,8-benzoflavone (7,8-BF) has been studied with the use of a dye-coupling technique and transformed Djungarian hamster DM15 fibroblasts. MC, EMS and 7,8-BF were found to exert a strong inhibitory effect on cell-to-cell dye transfer. BA and DMBA had the uncoupling activity only in 2 out of 4 experiments. B(e)P was not shown to affect LY transfer between DM15 cells. The uncoupling effect of MC, 7,8-BF and EMS (only when EMS used at the concentration of 600 μg/ ml but not 1000 μ/ ml) appeared reversible. The causes of failure to detect DMBA and B(e)P effects on gap junctions are discussed.",

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author = "Budunova, {Irene V.} and Mittelman, {Leonid A.} and Belitsky, {Gennady A.}",

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AU - Belitsky, Gennady A.

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N2 - The effect on permeability of gap junctions of complete powerful carcinogens, 3-methylcholanthrene (MC), 7, 12-dimethylbenz(a)anthracene (DMBA), ethyl methanesulfonate (EMS), and weak carcinogens, benz(a)anthracene (BA), benzo(e)pyrene (B(e)P) as well as the arylhydrolase inhibitor 7,8-benzoflavone (7,8-BF) has been studied with the use of a dye-coupling technique and transformed Djungarian hamster DM15 fibroblasts. MC, EMS and 7,8-BF were found to exert a strong inhibitory effect on cell-to-cell dye transfer. BA and DMBA had the uncoupling activity only in 2 out of 4 experiments. B(e)P was not shown to affect LY transfer between DM15 cells. The uncoupling effect of MC, 7,8-BF and EMS (only when EMS used at the concentration of 600 μg/ ml but not 1000 μ/ ml) appeared reversible. The causes of failure to detect DMBA and B(e)P effects on gap junctions are discussed.

AB - The effect on permeability of gap junctions of complete powerful carcinogens, 3-methylcholanthrene (MC), 7, 12-dimethylbenz(a)anthracene (DMBA), ethyl methanesulfonate (EMS), and weak carcinogens, benz(a)anthracene (BA), benzo(e)pyrene (B(e)P) as well as the arylhydrolase inhibitor 7,8-benzoflavone (7,8-BF) has been studied with the use of a dye-coupling technique and transformed Djungarian hamster DM15 fibroblasts. MC, EMS and 7,8-BF were found to exert a strong inhibitory effect on cell-to-cell dye transfer. BA and DMBA had the uncoupling activity only in 2 out of 4 experiments. B(e)P was not shown to affect LY transfer between DM15 cells. The uncoupling effect of MC, 7,8-BF and EMS (only when EMS used at the concentration of 600 μg/ ml but not 1000 μ/ ml) appeared reversible. The causes of failure to detect DMBA and B(e)P effects on gap junctions are discussed.

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The effect of complete carcinogens on intercellular transfer of lucifer yellow in fibroblast culture

Abstract

Keywords

ASJC Scopus subject areas

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