The effect of cyclopropenoid fatty acids on binding of aflatoxin B1 to nuclear DNA, RNA and protein of rat hepatocytes

D. G. Scarpelli, M. S. Rao, J. K. Reddy

Research output: Contribution to journalArticlepeer-review

Abstract

C18 and C19 fatty acids containing a cyclopropene ring (CPFA's) natural compounds in plants of the order Malvales have been shown to augment the cardiogenic effects of aflatoxin B1 (AFB1) and recently identified as a potent mitogen for hepatocytes. Experiments to ascertain whether CPFA's affect the binding of AFB1 to DNA, RNA and protein of rat liver cells were carried out. 50 gm male Sprague Dawley rats were fed a synthetic diet containing 500 ppm of CPFA's supplied as triglycerides in Sterculia foetida oil ad libitum for 2 wks. Comparable animals fed synthetic diet alone served as controls. Animals were injected I.P. (3μC/100 gm B.W.) with C14 ring labeled chromatographically pure AFB1 (Sp. Ac. 5.37 μC/μM) dissolved in N methylformamide and sacrificed 1 hr later. DNA, RNA and protein were extracted; DNA was further purified by treatment with pronase, RNA ase, and by density gradient centrifugation on CsCl2. These results corroborate earlier work that AFB1 binds to a greater degree to protein and RNA than to DNA. Ingestion of CPFA enhanced binding of AFB1 to protein by 39.9%, RNA 35.5% and DNA 37%. This effect of CPFA may be related to its potentiation of AFB1 induced hepatocarcinoma.

Original languageEnglish (US)
Pages (from-to)No. 655
JournalProceedings of the American Association for Cancer Research
Volume16
Issue number66
StatePublished - Jan 1 1975

ASJC Scopus subject areas

  • Medicine(all)

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