The effect of flow on the reduction of methylene blue (MB) and toluidine blue O (tbo) in the pulmonary circulation, ml. schulte

C. A. Dawson*, R. D. Bongard, S. Audi, J. H. Linehan

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

To determine whether the reduction of thiazine dyes on passage through the pulmonary circulation depends on the rate of reduction by the endothelium or on the dye delivery rate, we measured the reduction of MB and TBO in isolated rabbit lungs perfused over a range of flow rates. A mixture of dye and reference indicator (I albumin) was injected into the flowing perfusate. As the perfusate flow rate increased, the fraction of dye reduced by the lung decreased for both MB and TBO. The product of the association rate constant (kj and the amount of reductase on the endothelial surface (E) remained relatively constant for each dye over the range of flow rates. These results indicate that dye reduction by the endothelium was the rate limiting process and that the decrease in the reduced fraction with flow was mainly due to the decreased amount of time the dye was available for interaction with the endothelial surface. Methylene Blue Toluidine Blue O flow rate (ml/sec) 0.82 1.64 3.34 4.95 6.43 9.33 12.5 ±10.01(S.E.) ± 0.02 ± 0.06 ± 0.05 ± 0.09 ± 0.07 ± 0.09 reduced fraction (%) 46.6 33.0 21.4 12.0 84.7 76.6 71.1 ± 12.49 ± 2.62 ± 1.74 ± 2.12 ± 2.23 ±1.68 ± 2.71 kaE (ml/sec) 0.52 0.66 0.81 0.63 12.4 14.2 15.8 ± 0.03 0.07 ±0.08 ± 0.12 10.84 ± 0.73 ± 1.01 Supported by HL 2434 and the Apartment of Veterans' Affairs.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - Dec 1 1996

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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