Abstract
The K+/H+ antiporter nigericin inhibits the intercellular exchange of the fluorescent dye Lucifer Yellow between DM15-transformed fibroblasts derived from the Djungarian hamster. The efficacy of nigericin action was related to its concentration and time of incubation. The nigericin-induced uncoupling effect on gap junctions was reversible and was shown to be based on its ability to cause cystolic acidification. The effect of nigericin on dye-coupling in intact and 12-O-tetradecanoyl-phorbol-13-acetate (TPA) pretreated cells did not differ, indicating that the uncoupling effect of H+ on gap junctions in DM15 cells was not mediated by the TPA-dependent isoform of protein kinase C.
Original language | English (US) |
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Pages (from-to) | 63-73 |
Number of pages | 11 |
Journal | Cell Biology and Toxicology |
Volume | 8 |
Issue number | 1 |
DOIs | |
State | Published - Mar 1992 |
Keywords
- H
- gap junctions
- nigericin
- protein kinase C
ASJC Scopus subject areas
- Health, Toxicology and Mutagenesis
- Toxicology
- Cell Biology