The effect of K+/H+ antiporter nigericin on gap junction permeability

Irene V. Budunova*, Leonid A. Mittelman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


The K+/H+ antiporter nigericin inhibits the intercellular exchange of the fluorescent dye Lucifer Yellow between DM15-transformed fibroblasts derived from the Djungarian hamster. The efficacy of nigericin action was related to its concentration and time of incubation. The nigericin-induced uncoupling effect on gap junctions was reversible and was shown to be based on its ability to cause cystolic acidification. The effect of nigericin on dye-coupling in intact and 12-O-tetradecanoyl-phorbol-13-acetate (TPA) pretreated cells did not differ, indicating that the uncoupling effect of H+ on gap junctions in DM15 cells was not mediated by the TPA-dependent isoform of protein kinase C.

Original languageEnglish (US)
Pages (from-to)63-73
Number of pages11
JournalCell Biology and Toxicology
Issue number1
StatePublished - Mar 1992


  • H
  • gap junctions
  • nigericin
  • protein kinase C

ASJC Scopus subject areas

  • Toxicology
  • Cell Biology
  • Health, Toxicology and Mutagenesis


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