The effect of lipoproteins on human glioblastoma growth in vitro

Joseph R. Moskal*, Mark Sinnett, Paul L. Kornblith, Patrick LaSala, Daniel M. Levine, Thomas S. Parker, Harry Lander

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Experiments were performed using an established human glioblastoma cell line to determine the effect of lipoproteins on regulating their growth. It was found that synthetic and natural human high density lipoproteins (HDL) were effective in inhibiting tumor cell growth in a nontoxic, dose-dependent manner, and that the LD50 was 10-fold lower than that for normal rat astrocytes grown under identical conditions. In the presence of the antioxidant, glutathione, essentially all of the growth-inhibiting properties of HDL could be reversed suggesting that oxidized lipids from the HDL interacting with the plasma membranes of the glioblastoma cells were responsible for the growth-inhibiting effect observed. The markedly lower concentration of HDL required to inhibit glioblastoma cells in culture compared to normal astrocytes suggested that the mechanism of HDL-induced inhibition may be important for tumor growth in vivo. One possible mechanism under investigation is the possibility of HDL modulation of a membrane-associated, tumor-specific phosphatase.

Original languageEnglish (US)
Pages (from-to)169-181
Number of pages13
JournalMolecular and Chemical Neuropathology
Volume17
Issue number2
DOIs
StatePublished - Oct 1992

Keywords

  • Lipoproteins
  • autocrine growth control
  • glioblastoma growth regulation
  • high density
  • membrane-membrane interactions
  • receptor-mediated processes
  • regulation of cell-cycle by phosphatases

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology

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