The effect of the addition of adipose-derived mesenchymal stem cells to a meniscal repair in the avascular zone: An experimental study in rabbits

Miguel Ngel Ruiz-Ibán*, Jorge Díaz-Heredia, Ignacio García-Gómez, Fausto Gonzalez-Lizán, Elena Elías-Martín, Victor Abraira

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

Purpose: To determine whether adipose-derived mesenchymal stem cells (ASCs) affect the healing rate of meniscal lesions sutured in the avascular zone in rabbits. Methods: Four groups were used. In group A (n = 12) a short, 5-mm-long longitudinal lesion in the avascular zone of the anterior horn of the medial meniscus was created and immediately sutured. In group B (n = 8) the same short lesion was created but suture was delayed 3 weeks. In group C (n = 12) a larger, 15-mm-long lesion that spanned the whole meniscus was created and sutured immediately. In group D (n = 8) the same large lesion was sutured 3 weeks later. Both knees in each rabbit were used: 1 served as the control, and in the other, 1 × 10 5 allogeneic ASCs marked with bromodeoxyuridine were placed in the lesion immediately before suturing. The animals were killed at 12 weeks. Results: In group A (short lesion, acute repair) 6 of 12 ASC-treated menisci and 0 of 12 controls had some healing (P =.014). In group B (short lesion, delayed repair) 2 of 8 ASC-treated menisci and 1 of 8 controls had some healing (P =.5). In group C (long lesion, acute repair) 6 of 12 ASC-treated menisci and 0 of 12 controls had some healing (P =.014). In group D (long lesion, delayed repair) 4 of 8 ASC-treated menisci and 0 of 8 controls had some healing (P =.07). The addition of ASCs increased the healing rate (odds ratio, 32 [range, 3.69 to 277]; P =.002). The histologic analysis of the healed zones identified well-formed meniscal fibrocartilage with persistence of cells derived from the ASCs (immunolocated with anti-bromodeoxyuridine antibodies). Conclusions: Adding ASCs to a repair in the avascular zone of rabbit menisci increases the chances of healing. Healing is improved in small and larger lesions. When suture is delayed, the effect is not as evident. Clinical Relevance: In the future, ASCs might help in meniscal repair in the avascular zone.

Original languageEnglish (US)
Pages (from-to)1688-1696
Number of pages9
JournalArthroscopy - Journal of Arthroscopic and Related Surgery
Volume27
Issue number12
DOIs
StatePublished - Dec 2011
Externally publishedYes

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

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