The effect of the rate of hydrostatic pressure depressurization on cells in culture

Ellen Tworkoski, Matthew R Glucksberg, Mark Johnson*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Changes in hydrostatic pressure, at levels as low as 10 mm Hg, have been reported in some studies to alter cell function in vitro; however, other studies have found no detectable changes using similar methodologies. We here investigate the hypothesis that the rate of depressurization, rather than elevated hydrostatic pressure itself, may be responsible for these reported changes. Hydrostatic pressure (100 mm Hg above atmospheric pressure) was applied to bovine aortic endothelial cells (BAECs) and PC12 neuronal cells using pressurized gas for periods ranging from 3 hours to 9 days, and then the system was either slowly (~30 minutes) or rapidly (~5 seconds) depressurized. Cell viability, apoptosis, proliferation, and F-actin distribution were then assayed. Our results did not show significant differences between rapidly and slowly depressurized cells that would explain differences previously reported in the literature. Moreover, we found no detectable effect of elevated hydrostatic pressure (with slow depressurization) on any measured variables. Our results do not confirm the findings of other groups that modest increases in hydrostatic pressure affect cell function, but we are not able to explain their findings.

Original languageEnglish (US)
Article numbere0189890
JournalPloS one
Volume13
Issue number1
DOIs
StatePublished - Jan 1 2018

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Hydrostatic Pressure
Hydrostatic pressure
cell culture
Cell Culture Techniques
Atmospheric Pressure
PC12 Cells
Endothelial cells
atmospheric pressure
cells
endothelial cells
Atmospheric pressure
cell viability
actin
Actins
Cell Survival
apoptosis
Endothelial Cells
Gases
neurons
Cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

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The effect of the rate of hydrostatic pressure depressurization on cells in culture. / Tworkoski, Ellen; Glucksberg, Matthew R; Johnson, Mark.

In: PloS one, Vol. 13, No. 1, e0189890, 01.01.2018.

Research output: Contribution to journalArticle

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