TY - JOUR
T1 - The effect of vitamin C on oxygen radical-induced sister-chromatid exchanges
AU - Weitberg, Alan B.
AU - Weitzman, Sigmund A.
N1 - Funding Information:
Supported by an American Cancer Society Junior Faculty Fellowship and NIH grant CA-00962 (Dr. Weitberg), American Cancer Society CD-190 (Dr. Weitzman), Edwin Hiam and Edwin S. Webster Foundation.
PY - 1985/9
Y1 - 1985/9
N2 - We studied the effects of vitamin C (sodium ascorbate) on the genotoxicity of oxygen radicals to tissue culture cells. Chinese hamster ovary cells (CHO cells), when exposed to an enzymatic oxygen radical generating system (xanthine oxidase plus hypoxanthine), develop increased numbers of sister-chromatid exchanges (SCEs). Inclusion of ascorbate in these incubations resulted in significant, but variable effects. In some cases, ascorbate (<0.1 mM) was protective and fewer SCEs were produced. In others, significant augmentation of oxygen radical-induced SCEs occurred. These experiments illustrate the complexity of the interactions of ascorbate in biologic systems and the difficulty of predicting a desirable or harmful effect.
AB - We studied the effects of vitamin C (sodium ascorbate) on the genotoxicity of oxygen radicals to tissue culture cells. Chinese hamster ovary cells (CHO cells), when exposed to an enzymatic oxygen radical generating system (xanthine oxidase plus hypoxanthine), develop increased numbers of sister-chromatid exchanges (SCEs). Inclusion of ascorbate in these incubations resulted in significant, but variable effects. In some cases, ascorbate (<0.1 mM) was protective and fewer SCEs were produced. In others, significant augmentation of oxygen radical-induced SCEs occurred. These experiments illustrate the complexity of the interactions of ascorbate in biologic systems and the difficulty of predicting a desirable or harmful effect.
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U2 - 10.1016/0165-7992(85)90119-8
DO - 10.1016/0165-7992(85)90119-8
M3 - Article
C2 - 3839899
AN - SCOPUS:0022258868
SN - 0165-7992
VL - 144
SP - 23
EP - 26
JO - Mutation Research Letters
JF - Mutation Research Letters
IS - 1
ER -