The effects of exposure to unsaturated fatty acids on opiate receptors, prostaglandin E1 receptors, and adenylate cyclase activity of neuroblastoma x glioma hybrid cells

R. McGee, J. G. Kenimer

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

The effects of alterations in membrane fatty acid composition on receptors for opiates and prostaglandin E1 (PGE1) and on the activity of adenylate cyclase were studied in clonal neuroblastoma x glioma hybrid cells (NG108-15) following exposure to unsaturated fatty acids. Decreases in binding of [3H]etorphine and [3H]PGE1 were observed in cells exposed to arachidonate (20:4), linoleate (18:2), and oleate (18:1). The observed decreases in binding (as much as 80% decrease under some conditions) were primarily associated with changes in the number of binding sites. The effects of 20:4 and 18:2 were more rapid and greater than those of 18:1. The decreases in binding approximately paralleled the time (6-72 hr) and concentration-dependent enrichment of the membranes with the exogenously supplied fatty acids. However, when 20:4-treated cells were switched to normal culture medium the number of binding sites returned to control values much more rapidly than did the phospholipid fatty acid composition. This observation suggests that decreases in receptor number were a result of changes occurring during the pretreatment with fatty acids (e.g., a decrease in receptor synthesis, a decrease in receptor insertion into the membranes, or increased removal of the receptor from the membranes); the apparent number of receptors was not determined by the membrane fatty acid composition at the time binding was measured. In contrast, the adenylate cyclase activity of the cells was unaffected by even very large changes in phospholipid fatty acid composition. Likewise, the regulatory response to chronic exposure of the cells to PGE1 (decreases in both PGE1 receptors and adenylate cyclase activity) was not affected by simultaneous exposure to PGE1 and 20:4. Thus, the number of opiate and PGE1 receptors was selectively altered by exposure of the cells to unsaturated fatty acids.

Original languageEnglish (US)
Pages (from-to)360-368
Number of pages9
JournalMolecular pharmacology
Volume22
Issue number2
StatePublished - 1982

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

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