The effects of extremely low shear stress on cellular proliferation and neointimal thickening in the failing bypass graft

Shari L. Meyerson*, Christopher L. Skelly, Michael A. Curi, Umar M. Shakur, James E. Vosicky, Seymour Glagov, Lewis B. Schwartz

*Corresponding author for this work

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91 Scopus citations

Abstract

Objective: Previous studies demonstrating a correlation between low shear stress (τ = 5-15 dyne/cm2) and experimental vein graft neointimal thickening (NIT) support the role of low τ in vein graft failure. However, a simple linear relationship between low t and NIT would underestimate the degree of NIT evident in high-grade occlusive lesions of failing human vein grafts. In this study we used a new experimental model that maintains patency at low τ (< 2 dyne/cm2), to delineate possible deviations from linearity in the low τ → NIT hypothesis. Methods: Thirty-two New Zealand White rabbits underwent creation of a common carotid vein patch with a segment of ipsilateral external jugular vein. Very low τ was created in 13 patches by ligation of the distal common carotid artery, leaving the only outflow through a small muscular branch. Normal τ was created hi 11 patches by leaving the common carotid artery outflow intact. High τ was created in eight patches by ligation of the contralateral common carotid artery. Six patches were harvested after 2 weeks for measurement of cell cycle entry by proliferating cell nuclear antigen (PCNA) immunohistochemistry. The remaining 26 patches were harvested after 4 weeks, perfusion fixed, and excised for morphometric analysis. Results: Mean blood flow and τ at implantation ranged from 0.5 to 41 mL/min and 0.07 to 15 dyne/cm2, respectively. At the time of harvest, 30 of 32 patches remained patent, and the artificially created aberrations in blood flow were maintained (range, 0.7-41 mL/min). After 2 weeks PCNA immunohistochemistry showed a significantly higher level of cell cycling in patches exposed to low τ (40 ± 5 vs 1.6 ± 0.3 PCNA-positive cells per high-power field; P < .001), which is equivalent to approximately 20% of the total cells present. In patches harvested after 4 weeks, NIT ranged from 42 to 328 μm and significantly correlated with mean τ at implantation. Patches with very low τ exhibited histologic characteristics similar to those of failing human bypass grafts, including laminar thrombus and flow-limiting luminal stenosis. The relationship between τ and NIT was nonlinear in that extremely low τ (<; 2 dyne/cm2) resulted in NIT beyond that predicted by a simple linear correlation (P = .003). Conclusion: Extremely low τ (<; 2 dyne/cm2) stimulates high rates of smooth muscle cellular proliferation in arterialized vein patches. NIT is accelerated in these regions of low τ far beyond that predicted by a simple linear model. The nonlinear nature of the cellular proliferative response and NIT at τ less than 2 dyne/cm2 may explain the rapid progression of neointimal lesions in failing bypass grafts.

Original languageEnglish (US)
Pages (from-to)90-97
Number of pages8
JournalJournal of Vascular Surgery
Volume34
Issue number1
DOIs
StatePublished - Jan 1 2001

ASJC Scopus subject areas

  • Surgery
  • Cardiology and Cardiovascular Medicine

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    Meyerson, S. L., Skelly, C. L., Curi, M. A., Shakur, U. M., Vosicky, J. E., Glagov, S., & Schwartz, L. B. (2001). The effects of extremely low shear stress on cellular proliferation and neointimal thickening in the failing bypass graft. Journal of Vascular Surgery, 34(1), 90-97. https://doi.org/10.1067/mva.2001.114819