TY - JOUR
T1 - The effects of hyperoxia on pulmonary clearance of Pseudomonas aeruginosa
AU - Dunn, M. M.
AU - Smith, L. J.
N1 - Funding Information:
Received for publication 20 August 1985, and in revised form 13 November 1985. This work was supported by the American Lung Association, the Earl M. Baine Biomedical Research Fund, Northwestern University, and the Veterans Administration. We thank Anna Knap for excellent technical assistance. Please address requests for reprints to Dr. Marc M. Dunn, Northwestern University Medical Center, Pulmonary Section, Wesley-456, 250 East Superior Street, Chicago, Illinois 60611.
PY - 1986
Y1 - 1986
N2 - Among the toxic effects of hyperoxia may be impaired pulmonary clearance of gram-negative bacteria. To better define this effect, we exposed BALB/c mice to 100% O2 for 24, 48, or 72 hr and intrabronchially inoculated them with 106 Pseudomonas aeruginosa. Clearance was assessed 4 hr later by quantitative lung cultures of air- and O2-exposed mice. Clearance was first reduced at 48 and 72 hr in mice exposed to O2. To determine the mechanism responsible, we measured bronchoalveolar lavage neutrophil (PMN) counts and neutrophil chemotactic activity at 0, 2, and 4 hr after instillation of P. aeruginosa into mice first exposed to air or O2 for 48 hr. Air-exposed mice had more PMNs than did O2-exposed mice after challenge (13.3 ± 2.1 x 105 vs. 4.4 ± 0.6 x 105). There was no difference in neutrophil chemotactic activity between air- and O2-exposed mice at any time, although chemotactic activity increased in both groups after challenge. Our data suggest that hyperoxia impairs pulmonary clearance of P. aeruginosa by decreasing the influx of PMNs and that this effect is not due to diminished chemotactic activity in bronchoalveolar lavage fluid.
AB - Among the toxic effects of hyperoxia may be impaired pulmonary clearance of gram-negative bacteria. To better define this effect, we exposed BALB/c mice to 100% O2 for 24, 48, or 72 hr and intrabronchially inoculated them with 106 Pseudomonas aeruginosa. Clearance was assessed 4 hr later by quantitative lung cultures of air- and O2-exposed mice. Clearance was first reduced at 48 and 72 hr in mice exposed to O2. To determine the mechanism responsible, we measured bronchoalveolar lavage neutrophil (PMN) counts and neutrophil chemotactic activity at 0, 2, and 4 hr after instillation of P. aeruginosa into mice first exposed to air or O2 for 48 hr. Air-exposed mice had more PMNs than did O2-exposed mice after challenge (13.3 ± 2.1 x 105 vs. 4.4 ± 0.6 x 105). There was no difference in neutrophil chemotactic activity between air- and O2-exposed mice at any time, although chemotactic activity increased in both groups after challenge. Our data suggest that hyperoxia impairs pulmonary clearance of P. aeruginosa by decreasing the influx of PMNs and that this effect is not due to diminished chemotactic activity in bronchoalveolar lavage fluid.
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U2 - 10.1093/infdis/153.4.676
DO - 10.1093/infdis/153.4.676
M3 - Article
C2 - 3081661
AN - SCOPUS:0022621160
SN - 0022-1899
VL - 153
SP - 676
EP - 681
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 4
ER -
