TY - JOUR
T1 - The effects of inhaled budesonide on circulating eosinophil progenitors and their expression of cytokines after allergen challenge in subjects with atopic asthma
AU - Gauvreau, G. M.
AU - Wood, L. J.
AU - Sehmi, R.
AU - Watson, R. M.
AU - Dorman, S. C.
AU - Schleimer, R. P.
AU - Denburg, J. A.
AU - O'Byrne, P. M.
PY - 2000
Y1 - 2000
N2 - Allergen inhalation by dual responder subjects with atopic asthma is associated with an increase in circulating eosinophil/basophil colony-forming units (Eo/B CFU) and granulocyte-macrophage colony-stimulating factor (GM-CSF) immunolocalization in Eo/B colony cells grown in vitro. The current study examined the effect of the inhaled corticosteroid, budesonide, on the number of allergen-induced circulating eosinophils and Eo/B CFU, and immunolocalization of GM-CSF and interleukin-5 (IL-5) in Eo/B colony cells grown in vitro. Sixteen subjects with mild atopic asthma were treated for either 7 or 8 d with 200 μg inhaled budesonide or placebo twice a day. Peripheral blood was collected before and 24 h after allergen inhalation challenge and nonadherent mononuclear cells (NAMC) were grown in methylcellulose culture. Eo/B CFU were enumerated after 14 d in culture, and prepared on slides for immunocytochemistry. Budesonide attenuated the allergen-induced increase in circulating eosinophils (4.0 ± 0.4 × 105/ml versus 6.5 ± 0.7 × 105/ml, p = 0.0001), circulating Eo/B CFU (12.4 ± 2.3/106 NAMC versus 18.8 ± 4.6/106 NAMC, p = 0.05), and immunolocalization of GM-CSF in Eo/B colony cells (11.8 ± 1.9% positive versus 18.0 ± 2.2%, p = 0.01) but not immunolocalization of IL-5 (7.9 ± 1.4% versus 4.5 ± 0.6%, p > 0.05). Inhaled budesonide attenuated the number of allergen-induced circulating eosinophils and their progenitors grown in the presence of GM-CSF, which may partially be a result of regulating eosinophil progenitor expression of the autocrine growth factor GM-CSF.
AB - Allergen inhalation by dual responder subjects with atopic asthma is associated with an increase in circulating eosinophil/basophil colony-forming units (Eo/B CFU) and granulocyte-macrophage colony-stimulating factor (GM-CSF) immunolocalization in Eo/B colony cells grown in vitro. The current study examined the effect of the inhaled corticosteroid, budesonide, on the number of allergen-induced circulating eosinophils and Eo/B CFU, and immunolocalization of GM-CSF and interleukin-5 (IL-5) in Eo/B colony cells grown in vitro. Sixteen subjects with mild atopic asthma were treated for either 7 or 8 d with 200 μg inhaled budesonide or placebo twice a day. Peripheral blood was collected before and 24 h after allergen inhalation challenge and nonadherent mononuclear cells (NAMC) were grown in methylcellulose culture. Eo/B CFU were enumerated after 14 d in culture, and prepared on slides for immunocytochemistry. Budesonide attenuated the allergen-induced increase in circulating eosinophils (4.0 ± 0.4 × 105/ml versus 6.5 ± 0.7 × 105/ml, p = 0.0001), circulating Eo/B CFU (12.4 ± 2.3/106 NAMC versus 18.8 ± 4.6/106 NAMC, p = 0.05), and immunolocalization of GM-CSF in Eo/B colony cells (11.8 ± 1.9% positive versus 18.0 ± 2.2%, p = 0.01) but not immunolocalization of IL-5 (7.9 ± 1.4% versus 4.5 ± 0.6%, p > 0.05). Inhaled budesonide attenuated the number of allergen-induced circulating eosinophils and their progenitors grown in the presence of GM-CSF, which may partially be a result of regulating eosinophil progenitor expression of the autocrine growth factor GM-CSF.
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U2 - 10.1164/ajrccm.162.6.2001120
DO - 10.1164/ajrccm.162.6.2001120
M3 - Article
C2 - 11112128
AN - SCOPUS:0034526928
VL - 162
SP - 2139
EP - 2144
JO - American Review of Respiratory Disease
JF - American Review of Respiratory Disease
SN - 1073-449X
IS - 6
ER -