The effects of quercetin on α2-adrenoceptors in rat cerebral cortex

Zhi-Dong Ge*, A. W. Zhou, W. Wei

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

3H-clonidine (3H-CLO) was used to label α2-adrenoceptors in rat cerebral cortex and the effects of quercetin on α2-adrenoceptors in rat cerebral cortex were investigated. The binding of 3H-CLO to rat cerebral cortex was rapid (K1: 0.027 L·nmol-1·min-1) and readily reversed by 10 μmol·L-1 clonidine (K2:0.104 min-1). 3H-CLO saturation experiments indicated a single class of site with a K(D) of 5.87 ± 1.13 nmol·L-1 and B(max) of 160 ± 10 fmol·(mg protein)-1. The competition experiments with l-clonidine, l-epinephrine, l-norepinephrine and l-isoproterenol etc suggested that 3H-CLO binding site was α2-adrenoceptors. Quercetin (1 nmol·L-1~1 mmol·L-1) competitively inhibited the binding of 3H-CLO to α2-adrenoceptors and the K1 value of quercetin for α2-adrenoceptors was 5.73 ± 1.58 μmol·L-1.

Original languageEnglish (US)
Pages (from-to)383-386
Number of pages4
JournalChinese Pharmacological Bulletin
Volume11
Issue number5
StatePublished - 1995

Keywords

  • brain
  • clonidine
  • competitive binding
  • quercetin
  • radioligand assay
  • α-adrenoceptors

ASJC Scopus subject areas

  • Pharmacology

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