The effects of quercetin on α 2 -adrenoceptors in rat cerebral cortex

Zhi-Dong Ge*, A. W. Zhou, W. Wei

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

3 H-clonidine ( 3 H-CLO) was used to label α 2 -adrenoceptors in rat cerebral cortex and the effects of quercetin on α 2 -adrenoceptors in rat cerebral cortex were investigated. The binding of 3 H-CLO to rat cerebral cortex was rapid (K 1 : 0.027 L·nmol -1 ·min -1 ) and readily reversed by 10 μmol·L -1 clonidine (K 2 :0.104 min -1 ). 3 H-CLO saturation experiments indicated a single class of site with a K(D) of 5.87 ± 1.13 nmol·L -1 and B(max) of 160 ± 10 fmol·(mg protein) -1 . The competition experiments with l-clonidine, l-epinephrine, l-norepinephrine and l-isoproterenol etc suggested that 3 H-CLO binding site was α 2 -adrenoceptors. Quercetin (1 nmol·L -1 ~1 mmol·L -1 ) competitively inhibited the binding of 3 H-CLO to α 2 -adrenoceptors and the K 1 value of quercetin for α 2 -adrenoceptors was 5.73 ± 1.58 μmol·L -1 .

Original languageEnglish (US)
Pages (from-to)383-386
Number of pages4
JournalChinese Pharmacological Bulletin
Volume11
Issue number5
StatePublished - Jan 1 1995

Keywords

  • brain
  • clonidine
  • competitive binding
  • quercetin
  • radioligand assay
  • α -adrenoceptors

ASJC Scopus subject areas

  • Pharmacology

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