The emerging process of Top Down mass spectrometry for protein analysis: Biomarkers, protein-therapeutics, and achieving high throughput

John F. Kellie, John C. Tran, Ji Eun Lee, Dorothy R. Ahlf, Haylee M. Thomas, Ioanna Ntai, Adam D. Catherman, Kenneth R. Durbin, Leonid Zamdborg, Adaikkalam Vellaichamy, Paul M. Thomas, Neil L. Kelleher*

*Corresponding author for this work

Research output: Contribution to journalReview article

72 Scopus citations

Abstract

Top Down mass spectrometry (MS) has emerged as an alternative to common Bottom Up strategies for protein analysis. In the Top Down approach, intact proteins are fragmented directly in the mass spectrometer to achieve both protein identification and characterization, even capturing information on combinatorial post-translational modifications. Just in the past two years, Top Down MS has seen incremental advances in instrumentation and dedicated software, and has also experienced a major boost from refined separations of whole proteins in complex mixtures that have both high recovery and reproducibility. Combined with steadily advancing commercial MS instrumentation and data processing, a high-throughput workflow covering intact proteins and polypeptides up to 70 kDa is directly visible in the near future.

Original languageEnglish (US)
Pages (from-to)1532-1539
Number of pages8
JournalMolecular BioSystems
Volume6
Issue number9
DOIs
StatePublished - Sep 1 2010

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ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

Cite this

Kellie, J. F., Tran, J. C., Lee, J. E., Ahlf, D. R., Thomas, H. M., Ntai, I., Catherman, A. D., Durbin, K. R., Zamdborg, L., Vellaichamy, A., Thomas, P. M., & Kelleher, N. L. (2010). The emerging process of Top Down mass spectrometry for protein analysis: Biomarkers, protein-therapeutics, and achieving high throughput. Molecular BioSystems, 6(9), 1532-1539. https://doi.org/10.1039/c000896f