The emerging process of Top Down mass spectrometry for protein analysis: Biomarkers, protein-therapeutics, and achieving high throughput

John F. Kellie; John C. Tran; Ji Eun Lee; Dorothy R. Ahlf; Haylee M. Thomas; Ioanna Ntai; Adam D. Catherman; Kenneth R. Durbin; Leonid Zamdborg; Adaikkalam Vellaichamy; Paul M. Thomas; Neil L. Kelleher

doi:10.1039/c000896f

The emerging process of Top Down mass spectrometry for protein analysis: Biomarkers, protein-therapeutics, and achieving high throughput

John F. Kellie, John C. Tran, Ji Eun Lee, Dorothy R. Ahlf, Haylee M. Thomas, Ioanna Ntai, Adam D. Catherman, Kenneth R. Durbin, Leonid Zamdborg, Adaikkalam Vellaichamy, Paul M. Thomas, Neil L. Kelleher^*

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

84 Scopus citations

Abstract

Top Down mass spectrometry (MS) has emerged as an alternative to common Bottom Up strategies for protein analysis. In the Top Down approach, intact proteins are fragmented directly in the mass spectrometer to achieve both protein identification and characterization, even capturing information on combinatorial post-translational modifications. Just in the past two years, Top Down MS has seen incremental advances in instrumentation and dedicated software, and has also experienced a major boost from refined separations of whole proteins in complex mixtures that have both high recovery and reproducibility. Combined with steadily advancing commercial MS instrumentation and data processing, a high-throughput workflow covering intact proteins and polypeptides up to 70 kDa is directly visible in the near future.

Original language	English (US)
Pages (from-to)	1532-1539
Number of pages	8
Journal	Molecular bioSystems
Volume	6
Issue number	9
DOIs	https://doi.org/10.1039/c000896f
State	Published - Sep 2010

ASJC Scopus subject areas

Biotechnology
Molecular Biology

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Kellie, J. F., Tran, J. C., Lee, J. E., Ahlf, D. R., Thomas, H. M., Ntai, I., Catherman, A. D., Durbin, K. R., Zamdborg, L., Vellaichamy, A., Thomas, P. M., & Kelleher, N. L. (2010). The emerging process of Top Down mass spectrometry for protein analysis: Biomarkers, protein-therapeutics, and achieving high throughput. Molecular bioSystems, 6(9), 1532-1539. https://doi.org/10.1039/c000896f

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title = "The emerging process of Top Down mass spectrometry for protein analysis: Biomarkers, protein-therapeutics, and achieving high throughput",

abstract = "Top Down mass spectrometry (MS) has emerged as an alternative to common Bottom Up strategies for protein analysis. In the Top Down approach, intact proteins are fragmented directly in the mass spectrometer to achieve both protein identification and characterization, even capturing information on combinatorial post-translational modifications. Just in the past two years, Top Down MS has seen incremental advances in instrumentation and dedicated software, and has also experienced a major boost from refined separations of whole proteins in complex mixtures that have both high recovery and reproducibility. Combined with steadily advancing commercial MS instrumentation and data processing, a high-throughput workflow covering intact proteins and polypeptides up to 70 kDa is directly visible in the near future.",

author = "Kellie, {John F.} and Tran, {John C.} and Lee, {Ji Eun} and Ahlf, {Dorothy R.} and Thomas, {Haylee M.} and Ioanna Ntai and Catherman, {Adam D.} and Durbin, {Kenneth R.} and Leonid Zamdborg and Adaikkalam Vellaichamy and Thomas, {Paul M.} and Kelleher, {Neil L.}",

year = "2010",

month = sep,

doi = "10.1039/c000896f",

language = "English (US)",

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Kellie, JF, Tran, JC, Lee, JE, Ahlf, DR, Thomas, HM, Ntai, I, Catherman, AD, Durbin, KR, Zamdborg, L, Vellaichamy, A, Thomas, PM & Kelleher, NL 2010, 'The emerging process of Top Down mass spectrometry for protein analysis: Biomarkers, protein-therapeutics, and achieving high throughput', Molecular bioSystems, vol. 6, no. 9, pp. 1532-1539. https://doi.org/10.1039/c000896f

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AU - Kellie, John F.

AU - Tran, John C.

AU - Lee, Ji Eun

AU - Ahlf, Dorothy R.

AU - Thomas, Haylee M.

AU - Ntai, Ioanna

AU - Catherman, Adam D.

AU - Durbin, Kenneth R.

AU - Zamdborg, Leonid

AU - Vellaichamy, Adaikkalam

AU - Thomas, Paul M.

AU - Kelleher, Neil L.

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N2 - Top Down mass spectrometry (MS) has emerged as an alternative to common Bottom Up strategies for protein analysis. In the Top Down approach, intact proteins are fragmented directly in the mass spectrometer to achieve both protein identification and characterization, even capturing information on combinatorial post-translational modifications. Just in the past two years, Top Down MS has seen incremental advances in instrumentation and dedicated software, and has also experienced a major boost from refined separations of whole proteins in complex mixtures that have both high recovery and reproducibility. Combined with steadily advancing commercial MS instrumentation and data processing, a high-throughput workflow covering intact proteins and polypeptides up to 70 kDa is directly visible in the near future.

AB - Top Down mass spectrometry (MS) has emerged as an alternative to common Bottom Up strategies for protein analysis. In the Top Down approach, intact proteins are fragmented directly in the mass spectrometer to achieve both protein identification and characterization, even capturing information on combinatorial post-translational modifications. Just in the past two years, Top Down MS has seen incremental advances in instrumentation and dedicated software, and has also experienced a major boost from refined separations of whole proteins in complex mixtures that have both high recovery and reproducibility. Combined with steadily advancing commercial MS instrumentation and data processing, a high-throughput workflow covering intact proteins and polypeptides up to 70 kDa is directly visible in the near future.

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The emerging process of Top Down mass spectrometry for protein analysis: Biomarkers, protein-therapeutics, and achieving high throughput

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