The epididymal fat pad as a transplant site for minimal islet mass

Xiaojuan Chen, Xiaomin Zhang, Courtney Larson, Fei Chen, Hermann Kissler, Dixon B. Kaufman*

*Corresponding author for this work

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

The epididymal fat pad was evaluated as a site of islet transplantation in a syngeneic murine model of diabetes by comparing the transplant outcomes to that of islets transplanted intraportal. Mouse islets engrafted on the intra-abdominal epididymal fat pad ameliorated streptozotocin-induced hyperglycemia with similar efficacy as grafts implanted intraportally. Mice that received as few as 50 islets, either intraportal or in the epididymal fat pad, displayed similar glucose tolerance curves. Bioluminescence imaging and glucose measurement showed stable luminescence signals and blood glucose levels for over 5 months in both transplant sites using transgenic luciferase-positive islets. Prompt recurrent hyperglycemia occurred in all mice after removal of the epididymal fat pad bearing the islet graft. Histological examination of the grafts showed well-granulated insulin containing cells surrounded by healthy adipocytes. This study indicates that the epididymal fat pad maybe a useful islet transplant site in the mouse model for effective glycemic control.

Original languageEnglish (US)
Pages (from-to)122-125
Number of pages4
JournalTransplantation
Volume84
Issue number1
DOIs
StatePublished - Jul 2007

Keywords

  • Engraftment
  • Islet imaging
  • Islet transplantation

ASJC Scopus subject areas

  • Transplantation

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    Chen, X., Zhang, X., Larson, C., Chen, F., Kissler, H., & Kaufman, D. B. (2007). The epididymal fat pad as a transplant site for minimal islet mass. Transplantation, 84(1), 122-125. https://doi.org/10.1097/01.tp.0000266909.58117.e3