The ESCRT-III Protein CHMP1A Mediates Secretion of Sonic Hedgehog on a Distinctive Subtype of Extracellular Vesicles

Michael E. Coulter, Cristina M. Dorobantu, Gerrald A. Lodewijk, François Delalande, Sarah Cianferani, Vijay S. Ganesh, Richard S. Smith, Elaine T. Lim, C. Shan Xu, Song Pang, Eric T. Wong, Hart G.W. Lidov, Monica L. Calicchio, Edward Yang, Dilenny M. Gonzalez, Thorsten M. Schlaeger, Ganeshwaran H. Mochida, Harald Hess, Wei Chung Allen Lee, Maria K. LehtinenTomas Kirchhausen, David Haussler, Frank M.J. Jacobs*, Raphael Gaudin, Christopher A. Walsh

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

Endosomal sorting complex required for transport (ESCRT) complex proteins regulate biogenesis and release of extracellular vesicles (EVs), which enable cell-to-cell communication in the nervous system essential for development and adult function. We recently showed human loss-of-function (LOF) mutations in ESCRT-III member CHMP1A cause autosomal recessive microcephaly with pontocerebellar hypoplasia, but its mechanism was unclear. Here, we show Chmp1a is required for progenitor proliferation in mouse cortex and cerebellum and progenitor maintenance in human cerebral organoids. In Chmp1a null mice, this defect is associated with impaired sonic hedgehog (Shh) secretion and intraluminal vesicle (ILV) formation in multivesicular bodies (MVBs). Furthermore, we show CHMP1A is important for release of an EV subtype that contains AXL, RAB18, and TMED10 (ART) and SHH. Our findings show CHMP1A loss impairs secretion of SHH on ART-EVs, providing molecular mechanistic insights into the role of ESCRT proteins and EVs in the brain.

Original languageEnglish (US)
Pages (from-to)973-986.e8
JournalCell reports
Volume24
Issue number4
DOIs
StatePublished - Jul 24 2018
Externally publishedYes

Keywords

  • CHMP1A
  • ESCRT
  • extracellular vesicles
  • microcephaly
  • multivesicular body
  • neurodevelopment
  • sonic hedgehog

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint

Dive into the research topics of 'The ESCRT-III Protein CHMP1A Mediates Secretion of Sonic Hedgehog on a Distinctive Subtype of Extracellular Vesicles'. Together they form a unique fingerprint.

Cite this