TY - JOUR
T1 - The Ets-1 transcription factor is required for the development of natural killer cells in mice
AU - Barton, Kevin
AU - Muthusamy, Natarajan
AU - Fischer, Christopher
AU - Ting, Chao Nan
AU - Walunas, Theresa L.
AU - Lanier, Lewis L.
AU - Leiden, Jeffrey M.
N1 - Funding Information:
We thank T. Ley for the generous gift of the RW ES cells, P. Cresswell for the RMA-S cells, and C. Clendenin and K. Sigrist for help with the generation of the Ets-1-deficient mice. L. Gottschalk helped with the preparation of illustrations. P. Lawrey assisted with the preparation of the manuscript. This work was supported in part by a grant from the National Institute of Allergy and Infectious Diseases of the National Institutes of Health (to J. L.) and by the Cancer Center of the University of Chicago. DNAX is supported by Schering-Plough Corporation.
PY - 1998/10
Y1 - 1998/10
N2 - In this report we have investigated the role of the Ets-1 transcription factor in the differentiation of the NK cell lineage in mice. Splenic NK cells express high levels of Ets-1. Ets-1-deficient mice produced by gene targeting developed mature erythrocytes, monocytes, neutrophils, and T and B lymphocytes. However, spleens from the Ets-1-deficient mice contained significantly reduced numbers of natural killer (NK) cells, and splenocytes from these mice lacked detectable cytolytic activity against NK cell targets in vitro. Moreover, unlike wild-type animals, Ets-1-deficient mice developed tumors following subcutaneous injection of NK-susceptible RMA-S cells. These NK cell defects could not be correlated with defects in the expression of IL- 12, IL-15, and IL-18 or the IL-2 or IL-15 receptors. Thus, Ets-1 defines a novel transcriptional pathway that is required for the development of the NK cell lineage in mice.
AB - In this report we have investigated the role of the Ets-1 transcription factor in the differentiation of the NK cell lineage in mice. Splenic NK cells express high levels of Ets-1. Ets-1-deficient mice produced by gene targeting developed mature erythrocytes, monocytes, neutrophils, and T and B lymphocytes. However, spleens from the Ets-1-deficient mice contained significantly reduced numbers of natural killer (NK) cells, and splenocytes from these mice lacked detectable cytolytic activity against NK cell targets in vitro. Moreover, unlike wild-type animals, Ets-1-deficient mice developed tumors following subcutaneous injection of NK-susceptible RMA-S cells. These NK cell defects could not be correlated with defects in the expression of IL- 12, IL-15, and IL-18 or the IL-2 or IL-15 receptors. Thus, Ets-1 defines a novel transcriptional pathway that is required for the development of the NK cell lineage in mice.
UR - http://www.scopus.com/inward/record.url?scp=0032191918&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032191918&partnerID=8YFLogxK
U2 - 10.1016/S1074-7613(00)80638-X
DO - 10.1016/S1074-7613(00)80638-X
M3 - Article
C2 - 9806641
AN - SCOPUS:0032191918
VL - 9
SP - 555
EP - 563
JO - Immunity
JF - Immunity
SN - 1074-7613
IS - 4
ER -
