The expression of γ-aminobutyric acid and leu-enkephalin immunoreactivity in primary monolayer cultures of rat striatum

D. James Surmeier*, Hitoshi Kita, S. T. Kitai

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Primary monolayer cultures of rat striatum were examined for γ-aminobutyric acid (GABA) and leucine-enkephalin (L-ENK) immunoreactivity. Cultures were established on polycation-treated glass coverslips from the striata of gestational day 17 rat embryos using a serum and insulin-supplemented medium. The proportion of GABA-immunoreactive (GABA-IR) neurons increased during the first week in vitro from approximately one third to nearly one half and remained relatively constant thereafter. On the other hand, the proportion of L-ENK-IR neurons increased gradually over the culturing period, increasing from about one-fifth of the neurons initially to one-half after 3-4 weeks in vitro. The changes in the proportions of GABA- and L-ENK-IR neurons appeared to be largely a consequence of the death of non-immunoreactive neurons, not delayed expression or induction of GABA or L-ENK traits. Light microscopic analysis of somatic-proximal neuritic morphology led to a partitioning of the neuronal population into 4 groups. GABA- and L-ENK-IR groups were heterogeneous in this regard and differed only modestly.

Original languageEnglish (US)
Pages (from-to)265-282
Number of pages18
JournalDevelopmental Brain Research
Volume42
Issue number2
DOIs
StatePublished - Aug 1 1988

Keywords

  • Culture
  • Development
  • Immunocytochemistry
  • Leu-enkephalin
  • Striatum
  • γ-Aminobutyric acid

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Developmental Biology

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