The expression of acetylated microtubules during axonal and dendritic growth in cerebellar macroneurons which develop in vitro

Adriana Ferreira, Alfredo Cáceres*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

65 Scopus citations

Abstract

The distribution of acetylated microtubules in cerebellar macroneurons which develop in culture was studied with 6-11B-1, a monoclonal antibody specific for acetylated alpha-tubulin. In these neurons 6-11B-1 helps to define a subset of stable, colchicine-resistant, microtubules that during early neuronal development are exclusively localized in the neuron's axon. On the other hand, in mature neurons acetylated microtubules display a widespread distribution being localized in the axon and thick dendritic trunks, a phenomenon correlated with an increase in the levels of acetylated alpha-tubulin and colchicine-resistant microtubules. Taken collectively, these observations suggest that the acetylation of alpha-tubulin is important for differentiating microtubules during neurite growth; in young neurons this post-translational modification may contribute to determine a selective stabilization of microtubules accompanying axonal differentiation.

Original languageEnglish (US)
Pages (from-to)205-213
Number of pages9
JournalDevelopmental Brain Research
Volume49
Issue number2
DOIs
StatePublished - Oct 1 1989

Keywords

  • Acetylated alpha-tubulin
  • Cerebellum
  • Immunocytochemistry
  • Microtubule
  • Neurite growth
  • Stability
  • Tissue culture

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Developmental Biology

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