The expression of CMP-NeuAc: Galβ1,4GlcNAc α2,6 sialyltransferase [EC 2.4.99.1] and glycoproteins bearing α2,6-linked sialic acids in human brain tumours

Hirotaka Yamamoto, Yoichi Kaneko, David Vandermulen, Donna Kersey, Edward Mkrdichian, Leonard Cerullo, Jan Leestma, Joseph R. Moskal*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

The expression of CMP-NeuAc: Galβ1,4GlcNAc α2,6 sialyltransferase (α2,6-ST) [EC 2.4.99.1] and glycoproteins bearing α2,6-linked sialic acids were examined in primary human brain tumours and cell lines. 79% (19/24) of the meningiomas expressed α2,6-ST mRNA, 42% (10/24) of which showed very high expression. α2,6-ST mRNA expression was undetectable in normal brain tissue. In contrast, only 1/13 of the gliomas examined expressed detectable α2,6-ST mRNA. Metastases to the brain did not express measurable amounts of α2,6-ST mRNA. Less expression was found in malignant (i.e., anaplastic) compared to benign (i.e. meningothelial) meningiomas. Two-dimensional SDS-PAGE of glioma and meningioma proteins, followed by Sambucus nigra lectin staining, revealed the presence of a glycoprotein bearing α2,6-linked sialic acids, Mr=53 kDa and a pI=7.0 (MEN-1) that appeared in all seven of the meningiomas examined, but was expressed at barely detectable levels, if at all, in seven out of the seven glioblastomas examined. Thus, decreased α2,6-ST expression may play a role in the aggressive nature of anaplastic meningiomas, but appears to be virtually absent in all tumours of glial origin.

Original languageEnglish (US)
Pages (from-to)848-856
Number of pages9
JournalGlycoconjugate Journal
Volume12
Issue number6
DOIs
StatePublished - Dec 1995

Keywords

  • Sambucus nigra lectin
  • glioblastoma
  • meningioma
  • sialyltransferase
  • α2,6-linked sialic acids

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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