TY - JOUR
T1 - The expression of Galβ1,4GlcNAc α2,6 sialyltransferase and α2,6-linked sialoglycoconjugates in human brain tumors
AU - Kaneko, Yoichi
AU - Yamamoto, Hirotaka
AU - Kersey, Donna S.
AU - Colley, Karen J.
AU - Leestma, Jan E.
AU - Moskal, Joseph R.
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1996
Y1 - 1996
N2 - CMP-NeuAc: Galβ1,4GlcNAc α2,6 sialyltransferase (α2,6-ST) [EC 2.4.99.1] is developmentally regulated, shows a high degree of tissue specificity, and appears to play a role in oncogenic transformation and metastasis. In the present study, we have performed the first detailed analysis of the expression of α2,6-ST and α2,6-linked sialoglycoconjugates in human brain tumors. We used a polyclonal, monospecific anti-rat α2,6-ST antibody and the α2,6-linked sialic acid-specific lectin, Sambucus nigra agglutinin (SNA) for histochemical studies, and a human α2,6-ST-specific cDNA probe for Northern analysis. Meningiomas, chordomas and craniopharyngiomas frequently expressed α2,6-ST and α2,6-linked sialoglycoconjugates. Among the different meningioma subtypes, meningothelial meningiomas stained more strongly with both anti-α2,6-ST antibody and SNA than the fibroblastic and anaplastic meningiomas. On the other hand, all tumors of glial origin and medulloblastomas were virtually devoid of either α2,6-ST or α2,6-linked sialoglycoconjugate expression. Moreover, very weak to negligible expression of both α2,6-ST and α2,6-linked sialoglycoconjugates was observed in brain metastases. In conclusion, α2,6-ST and α2,6-linked sialoglycoconjugate expression is associated with non-neuroectodermal epithelial-like tumors.
AB - CMP-NeuAc: Galβ1,4GlcNAc α2,6 sialyltransferase (α2,6-ST) [EC 2.4.99.1] is developmentally regulated, shows a high degree of tissue specificity, and appears to play a role in oncogenic transformation and metastasis. In the present study, we have performed the first detailed analysis of the expression of α2,6-ST and α2,6-linked sialoglycoconjugates in human brain tumors. We used a polyclonal, monospecific anti-rat α2,6-ST antibody and the α2,6-linked sialic acid-specific lectin, Sambucus nigra agglutinin (SNA) for histochemical studies, and a human α2,6-ST-specific cDNA probe for Northern analysis. Meningiomas, chordomas and craniopharyngiomas frequently expressed α2,6-ST and α2,6-linked sialoglycoconjugates. Among the different meningioma subtypes, meningothelial meningiomas stained more strongly with both anti-α2,6-ST antibody and SNA than the fibroblastic and anaplastic meningiomas. On the other hand, all tumors of glial origin and medulloblastomas were virtually devoid of either α2,6-ST or α2,6-linked sialoglycoconjugate expression. Moreover, very weak to negligible expression of both α2,6-ST and α2,6-linked sialoglycoconjugates was observed in brain metastases. In conclusion, α2,6-ST and α2,6-linked sialoglycoconjugate expression is associated with non-neuroectodermal epithelial-like tumors.
KW - Glioma
KW - Glycosyltransferase
KW - Meningioma
KW - Metastasis
KW - Sambucus nigra agglutinin
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UR - http://www.scopus.com/inward/citedby.url?scp=0030071858&partnerID=8YFLogxK
U2 - 10.1007/s004010050427
DO - 10.1007/s004010050427
M3 - Article
C2 - 8834541
AN - SCOPUS:0030071858
VL - 91
SP - 284
EP - 292
JO - Acta Neuropathologica
JF - Acta Neuropathologica
SN - 0001-6322
IS - 3
ER -