The extracellular loop of pendrin and prestin modulates their voltage-sensing property

Makoto F. Kuwabara, Koichiro Wasano, Satoe Takahashi, Justin Bodner, Tomotaka Komori, Sotaro Uemura, Jing Zheng, Tomohiro Shima, Kazuaki Homma

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Pendrin and prestin belong to the solute carrier 26 (SLC26) family of anion transporters. Prestin is unique among the SLC26 family members in that it displays voltage-driven motor activity (electromotility) and concurrent gating currents that manifest as nonlinear cell membrane electrical capacitance (nonlinear capacitance (NLC)). Although the anion transport mechanism of the SLC26 proteins has begun to be elucidated, the molecular mechanism of electromotility, which is thought to have evolved from an ancestral ion transport mechanism, still remains largely elusive. Here, we demonstrate that pendrin also exhibits large NLC and that charged residues present in one of the extracellular loops of pendrin and prestin play significant roles in setting the voltage-operating points of NLC. Our results suggest that the molecular mechanism responsible for sensing voltage is not unique to prestin among the members of the SLC26 family and that this voltage-sensing mechanism works independently of the anion transport mechanism.

Original languageEnglish (US)
Pages (from-to)9970-9980
Number of pages11
JournalJournal of Biological Chemistry
Volume293
Issue number26
DOIs
StatePublished - Jan 1 2018

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Anions
Capacitance
Electric Capacitance
Electric potential
Ion Transport
Motor Activity
Cell membranes
Cell Membrane
Ions
Solute Carrier Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Kuwabara, Makoto F. ; Wasano, Koichiro ; Takahashi, Satoe ; Bodner, Justin ; Komori, Tomotaka ; Uemura, Sotaro ; Zheng, Jing ; Shima, Tomohiro ; Homma, Kazuaki. / The extracellular loop of pendrin and prestin modulates their voltage-sensing property. In: Journal of Biological Chemistry. 2018 ; Vol. 293, No. 26. pp. 9970-9980.
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Kuwabara, MF, Wasano, K, Takahashi, S, Bodner, J, Komori, T, Uemura, S, Zheng, J, Shima, T & Homma, K 2018, 'The extracellular loop of pendrin and prestin modulates their voltage-sensing property' Journal of Biological Chemistry, vol. 293, no. 26, pp. 9970-9980. https://doi.org/10.1074/jbc.RA118.001831

The extracellular loop of pendrin and prestin modulates their voltage-sensing property. / Kuwabara, Makoto F.; Wasano, Koichiro; Takahashi, Satoe; Bodner, Justin; Komori, Tomotaka; Uemura, Sotaro; Zheng, Jing; Shima, Tomohiro; Homma, Kazuaki.

In: Journal of Biological Chemistry, Vol. 293, No. 26, 01.01.2018, p. 9970-9980.

Research output: Contribution to journalArticle

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AU - Kuwabara, Makoto F.

AU - Wasano, Koichiro

AU - Takahashi, Satoe

AU - Bodner, Justin

AU - Komori, Tomotaka

AU - Uemura, Sotaro

AU - Zheng, Jing

AU - Shima, Tomohiro

AU - Homma, Kazuaki

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Kuwabara MF, Wasano K, Takahashi S, Bodner J, Komori T, Uemura S et al. The extracellular loop of pendrin and prestin modulates their voltage-sensing property. Journal of Biological Chemistry. 2018 Jan 1;293(26):9970-9980. https://doi.org/10.1074/jbc.RA118.001831