The extracellular matrix controls gap junction protein expression and function in postnatal hippocampal neural progenitor cells

Sophie Imbeault, Lianne G. Gauvin, Hadi Daood Toeg, Alexandra Pettit, Catherine D. Sorbara, Lamiaa Migahed, Rebecca DesRoches, A. Sheila Menzies, Kiyomasa Nishii, David L. Paul, Alexander M. Simon, Steffany A.L. Bennett*

*Corresponding author for this work

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Background: Gap junction protein and extracellular matrix signalling systems act in concert to influence developmental specification of neural stem and progenitor cells. It is not known how these two signalling systems interact. Here, we examined the role of ECM components in regulating connexin expression and function in postnatal hippocampal progenitor cells. Results: We found that Cx26, Cx29, Cx30, Cx37, Cx40, Cx43, Cx45, and Cx47 mRNA and protein but only Cx32 and Cx36 mRNA are detected in distinct neural progenitor cell populations cultured in the absence of exogenous ECM. Multipotential Type 1 cells express Cx26, Cx30, and Cx43 protein. Their Type 2a progeny but not Type 2b and 3 neuronally committed progenitor cells additionally express Cx37, Cx40, and Cx45. Cx29 and Cx47 protein is detected in early oligodendrocyte progenitors and mature oligodendrocytes respectively. Engagement with a laminin substrate markedly increases Cx26 protein expression, decreases Cx40, Cx43, Cx45, and Cx47 protein expression, and alters subcellular localization of Cx30. These changes are associated with decreased neurogenesis. Further, laminin elicits the appearance of Cx32 protein in early oligodendrocyte progenitors and Cx36 protein in immature neurons. These changes impact upon functional connexin-mediated hemichannel activity but not gap junctional intercellular communication. Conclusion: Together, these findings demonstrate a new role for extracellular matrix-cell interaction, specifically laminin, in the regulation of intrinsic connexin expression and function in postnatal neural progenitor cells.

Original languageEnglish (US)
Article number13
JournalBMC Neuroscience
Volume10
DOIs
StatePublished - Feb 24 2009

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Connexins
Extracellular Matrix
Stem Cells
Connexin 43
Oligodendroglia
Laminin
Proteins
Messenger RNA
Neural Stem Cells
Neurogenesis
Cell Communication
Neurons
Population

ASJC Scopus subject areas

  • Neuroscience(all)
  • Cellular and Molecular Neuroscience

Cite this

Imbeault, S., Gauvin, L. G., Toeg, H. D., Pettit, A., Sorbara, C. D., Migahed, L., ... Bennett, S. A. L. (2009). The extracellular matrix controls gap junction protein expression and function in postnatal hippocampal neural progenitor cells. BMC Neuroscience, 10, [13]. https://doi.org/10.1186/1471-2202-10-13
Imbeault, Sophie ; Gauvin, Lianne G. ; Toeg, Hadi Daood ; Pettit, Alexandra ; Sorbara, Catherine D. ; Migahed, Lamiaa ; DesRoches, Rebecca ; Menzies, A. Sheila ; Nishii, Kiyomasa ; Paul, David L. ; Simon, Alexander M. ; Bennett, Steffany A.L. / The extracellular matrix controls gap junction protein expression and function in postnatal hippocampal neural progenitor cells. In: BMC Neuroscience. 2009 ; Vol. 10.
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abstract = "Background: Gap junction protein and extracellular matrix signalling systems act in concert to influence developmental specification of neural stem and progenitor cells. It is not known how these two signalling systems interact. Here, we examined the role of ECM components in regulating connexin expression and function in postnatal hippocampal progenitor cells. Results: We found that Cx26, Cx29, Cx30, Cx37, Cx40, Cx43, Cx45, and Cx47 mRNA and protein but only Cx32 and Cx36 mRNA are detected in distinct neural progenitor cell populations cultured in the absence of exogenous ECM. Multipotential Type 1 cells express Cx26, Cx30, and Cx43 protein. Their Type 2a progeny but not Type 2b and 3 neuronally committed progenitor cells additionally express Cx37, Cx40, and Cx45. Cx29 and Cx47 protein is detected in early oligodendrocyte progenitors and mature oligodendrocytes respectively. Engagement with a laminin substrate markedly increases Cx26 protein expression, decreases Cx40, Cx43, Cx45, and Cx47 protein expression, and alters subcellular localization of Cx30. These changes are associated with decreased neurogenesis. Further, laminin elicits the appearance of Cx32 protein in early oligodendrocyte progenitors and Cx36 protein in immature neurons. These changes impact upon functional connexin-mediated hemichannel activity but not gap junctional intercellular communication. Conclusion: Together, these findings demonstrate a new role for extracellular matrix-cell interaction, specifically laminin, in the regulation of intrinsic connexin expression and function in postnatal neural progenitor cells.",
author = "Sophie Imbeault and Gauvin, {Lianne G.} and Toeg, {Hadi Daood} and Alexandra Pettit and Sorbara, {Catherine D.} and Lamiaa Migahed and Rebecca DesRoches and Menzies, {A. Sheila} and Kiyomasa Nishii and Paul, {David L.} and Simon, {Alexander M.} and Bennett, {Steffany A.L.}",
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Imbeault, S, Gauvin, LG, Toeg, HD, Pettit, A, Sorbara, CD, Migahed, L, DesRoches, R, Menzies, AS, Nishii, K, Paul, DL, Simon, AM & Bennett, SAL 2009, 'The extracellular matrix controls gap junction protein expression and function in postnatal hippocampal neural progenitor cells', BMC Neuroscience, vol. 10, 13. https://doi.org/10.1186/1471-2202-10-13

The extracellular matrix controls gap junction protein expression and function in postnatal hippocampal neural progenitor cells. / Imbeault, Sophie; Gauvin, Lianne G.; Toeg, Hadi Daood; Pettit, Alexandra; Sorbara, Catherine D.; Migahed, Lamiaa; DesRoches, Rebecca; Menzies, A. Sheila; Nishii, Kiyomasa; Paul, David L.; Simon, Alexander M.; Bennett, Steffany A.L.

In: BMC Neuroscience, Vol. 10, 13, 24.02.2009.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The extracellular matrix controls gap junction protein expression and function in postnatal hippocampal neural progenitor cells

AU - Imbeault, Sophie

AU - Gauvin, Lianne G.

AU - Toeg, Hadi Daood

AU - Pettit, Alexandra

AU - Sorbara, Catherine D.

AU - Migahed, Lamiaa

AU - DesRoches, Rebecca

AU - Menzies, A. Sheila

AU - Nishii, Kiyomasa

AU - Paul, David L.

AU - Simon, Alexander M.

AU - Bennett, Steffany A.L.

PY - 2009/2/24

Y1 - 2009/2/24

N2 - Background: Gap junction protein and extracellular matrix signalling systems act in concert to influence developmental specification of neural stem and progenitor cells. It is not known how these two signalling systems interact. Here, we examined the role of ECM components in regulating connexin expression and function in postnatal hippocampal progenitor cells. Results: We found that Cx26, Cx29, Cx30, Cx37, Cx40, Cx43, Cx45, and Cx47 mRNA and protein but only Cx32 and Cx36 mRNA are detected in distinct neural progenitor cell populations cultured in the absence of exogenous ECM. Multipotential Type 1 cells express Cx26, Cx30, and Cx43 protein. Their Type 2a progeny but not Type 2b and 3 neuronally committed progenitor cells additionally express Cx37, Cx40, and Cx45. Cx29 and Cx47 protein is detected in early oligodendrocyte progenitors and mature oligodendrocytes respectively. Engagement with a laminin substrate markedly increases Cx26 protein expression, decreases Cx40, Cx43, Cx45, and Cx47 protein expression, and alters subcellular localization of Cx30. These changes are associated with decreased neurogenesis. Further, laminin elicits the appearance of Cx32 protein in early oligodendrocyte progenitors and Cx36 protein in immature neurons. These changes impact upon functional connexin-mediated hemichannel activity but not gap junctional intercellular communication. Conclusion: Together, these findings demonstrate a new role for extracellular matrix-cell interaction, specifically laminin, in the regulation of intrinsic connexin expression and function in postnatal neural progenitor cells.

AB - Background: Gap junction protein and extracellular matrix signalling systems act in concert to influence developmental specification of neural stem and progenitor cells. It is not known how these two signalling systems interact. Here, we examined the role of ECM components in regulating connexin expression and function in postnatal hippocampal progenitor cells. Results: We found that Cx26, Cx29, Cx30, Cx37, Cx40, Cx43, Cx45, and Cx47 mRNA and protein but only Cx32 and Cx36 mRNA are detected in distinct neural progenitor cell populations cultured in the absence of exogenous ECM. Multipotential Type 1 cells express Cx26, Cx30, and Cx43 protein. Their Type 2a progeny but not Type 2b and 3 neuronally committed progenitor cells additionally express Cx37, Cx40, and Cx45. Cx29 and Cx47 protein is detected in early oligodendrocyte progenitors and mature oligodendrocytes respectively. Engagement with a laminin substrate markedly increases Cx26 protein expression, decreases Cx40, Cx43, Cx45, and Cx47 protein expression, and alters subcellular localization of Cx30. These changes are associated with decreased neurogenesis. Further, laminin elicits the appearance of Cx32 protein in early oligodendrocyte progenitors and Cx36 protein in immature neurons. These changes impact upon functional connexin-mediated hemichannel activity but not gap junctional intercellular communication. Conclusion: Together, these findings demonstrate a new role for extracellular matrix-cell interaction, specifically laminin, in the regulation of intrinsic connexin expression and function in postnatal neural progenitor cells.

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