Abstract
The dimorphic bacterium Caulobacter crescentus has evolved marked phenotypic changes during its 50-year history of culture in the laboratory environment, providing an excellent system for the study of natural selection and phenotypic microevolution in prokaryotes. Combining whole-genome sequencing with classical molecular genetic tools, we have comprehensively mapped a set of polymorphisms underlying multiple derived phenotypes, several of which arose independently in separate strain lineages. The genetic basis of phenotypic differences in growth rate, mucoidy, adhesion, sedimentation, phage susceptibility, and stationary-phase survival between C. crescentus strain CB15 and its derivative NA1000 is determined by coding, regulatory, and insertion/deletion polymorphisms at five chromosomal loci. This study evidences multiple genetic mechanisms of bacterial evolution as driven by selection for growth and survival in a new selective environment and identifies a common polymorphic locus, zwf, between lab-adapted C. crescentus and clinical isolates of Pseudomonas aeruginosa that have adapted to a human host during chronic infection.
| Language | English (US) |
|---|---|
| Pages | 3678-3688 |
| Number of pages | 11 |
| Journal | Journal of bacteriology |
| Volume | 192 |
| Issue number | 14 |
| DOIs | |
| State | Published - Jul 1 2010 |
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ASJC Scopus subject areas
- Microbiology
- Molecular Biology
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The genetic basis of laboratory adaptation in Caulobacter crescentus. / Marks, Melissa E.; Castro-Rojas, Cyd Marie; Teiling, Clotilde; Du, Lei; Kapatral, Vinayak; Walunas, Theresa L.; Crosson, Sean.
In: Journal of bacteriology, Vol. 192, No. 14, 01.07.2010, p. 3678-3688.Research output: Contribution to journal › Article
TY - JOUR
T1 - The genetic basis of laboratory adaptation in Caulobacter crescentus
AU - Marks,Melissa E.
AU - Castro-Rojas,Cyd Marie
AU - Teiling,Clotilde
AU - Du,Lei
AU - Kapatral,Vinayak
AU - Walunas,Theresa L.
AU - Crosson,Sean
PY - 2010/7/1
Y1 - 2010/7/1
N2 - The dimorphic bacterium Caulobacter crescentus has evolved marked phenotypic changes during its 50-year history of culture in the laboratory environment, providing an excellent system for the study of natural selection and phenotypic microevolution in prokaryotes. Combining whole-genome sequencing with classical molecular genetic tools, we have comprehensively mapped a set of polymorphisms underlying multiple derived phenotypes, several of which arose independently in separate strain lineages. The genetic basis of phenotypic differences in growth rate, mucoidy, adhesion, sedimentation, phage susceptibility, and stationary-phase survival between C. crescentus strain CB15 and its derivative NA1000 is determined by coding, regulatory, and insertion/deletion polymorphisms at five chromosomal loci. This study evidences multiple genetic mechanisms of bacterial evolution as driven by selection for growth and survival in a new selective environment and identifies a common polymorphic locus, zwf, between lab-adapted C. crescentus and clinical isolates of Pseudomonas aeruginosa that have adapted to a human host during chronic infection.
AB - The dimorphic bacterium Caulobacter crescentus has evolved marked phenotypic changes during its 50-year history of culture in the laboratory environment, providing an excellent system for the study of natural selection and phenotypic microevolution in prokaryotes. Combining whole-genome sequencing with classical molecular genetic tools, we have comprehensively mapped a set of polymorphisms underlying multiple derived phenotypes, several of which arose independently in separate strain lineages. The genetic basis of phenotypic differences in growth rate, mucoidy, adhesion, sedimentation, phage susceptibility, and stationary-phase survival between C. crescentus strain CB15 and its derivative NA1000 is determined by coding, regulatory, and insertion/deletion polymorphisms at five chromosomal loci. This study evidences multiple genetic mechanisms of bacterial evolution as driven by selection for growth and survival in a new selective environment and identifies a common polymorphic locus, zwf, between lab-adapted C. crescentus and clinical isolates of Pseudomonas aeruginosa that have adapted to a human host during chronic infection.
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U2 - 10.1128/JB.00255-10
DO - 10.1128/JB.00255-10
M3 - Article
VL - 192
SP - 3678
EP - 3688
JO - Journal of Bacteriology
T2 - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 14
ER -