The genetic basis of laboratory adaptation in Caulobacter crescentus

Melissa E. Marks, Cyd Marie Castro-Rojas, Clotilde Teiling, Lei Du, Vinayak Kapatral, Theresa L. Walunas, Sean Crosson

Research output: Contribution to journalArticle

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Abstract

The dimorphic bacterium Caulobacter crescentus has evolved marked phenotypic changes during its 50-year history of culture in the laboratory environment, providing an excellent system for the study of natural selection and phenotypic microevolution in prokaryotes. Combining whole-genome sequencing with classical molecular genetic tools, we have comprehensively mapped a set of polymorphisms underlying multiple derived phenotypes, several of which arose independently in separate strain lineages. The genetic basis of phenotypic differences in growth rate, mucoidy, adhesion, sedimentation, phage susceptibility, and stationary-phase survival between C. crescentus strain CB15 and its derivative NA1000 is determined by coding, regulatory, and insertion/deletion polymorphisms at five chromosomal loci. This study evidences multiple genetic mechanisms of bacterial evolution as driven by selection for growth and survival in a new selective environment and identifies a common polymorphic locus, zwf, between lab-adapted C. crescentus and clinical isolates of Pseudomonas aeruginosa that have adapted to a human host during chronic infection.

LanguageEnglish (US)
Pages3678-3688
Number of pages11
JournalJournal of bacteriology
Volume192
Issue number14
DOIs
StatePublished - Jul 1 2010

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Caulobacter crescentus
Survival
Genetic Selection
Growth
Bacteriophages
Pseudomonas aeruginosa
Molecular Biology
Genome
Bacteria
Phenotype
Infection

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Marks, M. E., Castro-Rojas, C. M., Teiling, C., Du, L., Kapatral, V., Walunas, T. L., & Crosson, S. (2010). The genetic basis of laboratory adaptation in Caulobacter crescentus. Journal of bacteriology, 192(14), 3678-3688. DOI: 10.1128/JB.00255-10
Marks, Melissa E. ; Castro-Rojas, Cyd Marie ; Teiling, Clotilde ; Du, Lei ; Kapatral, Vinayak ; Walunas, Theresa L. ; Crosson, Sean. / The genetic basis of laboratory adaptation in Caulobacter crescentus. In: Journal of bacteriology. 2010 ; Vol. 192, No. 14. pp. 3678-3688
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Marks, ME, Castro-Rojas, CM, Teiling, C, Du, L, Kapatral, V, Walunas, TL & Crosson, S 2010, 'The genetic basis of laboratory adaptation in Caulobacter crescentus' Journal of bacteriology, vol 192, no. 14, pp. 3678-3688. DOI: 10.1128/JB.00255-10

The genetic basis of laboratory adaptation in Caulobacter crescentus. / Marks, Melissa E.; Castro-Rojas, Cyd Marie; Teiling, Clotilde; Du, Lei; Kapatral, Vinayak; Walunas, Theresa L.; Crosson, Sean.

In: Journal of bacteriology, Vol. 192, No. 14, 01.07.2010, p. 3678-3688.

Research output: Contribution to journalArticle

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Marks ME, Castro-Rojas CM, Teiling C, Du L, Kapatral V, Walunas TL et al. The genetic basis of laboratory adaptation in Caulobacter crescentus. Journal of bacteriology. 2010 Jul 1;192(14):3678-3688. Available from, DOI: 10.1128/JB.00255-10