The hemicochlea preparation of the guinea pig and other mammalian cochleae

Ingo Ulrik Teudt, Claus Peter Richter*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

The hemicochlea and its slice preparation is a novel method that allows access to various cochlear structures without the physical distortion that typically occurs from tissue dehydration during the embedding process. Therefore, the hemicochlea preparation provides an excellent model to study (1) cochlear morphology during cochlear development, (2) malformation caused by genetic defects, (3) changes related to diseases, (4) sensory physiology, (5) cochlear micromechanics, and (6) the expression of proteins by immunohistochemistry. This paper describes in detail the method of slicing hemicochleae for different mammalian species, including mice, rats, gerbils, guinea pigs, pigs, and human temporal bones. Furthermore, guinea pig cochleae are used as an example to provide cochlear dimensions of important anatomical structures. The values obtained in eight guinea pig hemicochleae are compared to published values, and upon review, discrepancies do exist. For example, gelatinous structures, such as the tectorial membrane, appear larger in the hemicochlea when compared to traditional embedding. Dimensions obtained for selected cochlear structures at different locations along the guinea pig cochleae provide an improved basis for cochlear models.

Original languageEnglish (US)
Pages (from-to)187-197
Number of pages11
JournalJournal of Neuroscience Methods
Volume162
Issue number1-2
DOIs
StatePublished - May 15 2007

Keywords

  • Basilar membrane
  • Guinea pig
  • Hemicochlea
  • Method
  • Morphology
  • Tectorial membrane

ASJC Scopus subject areas

  • Neuroscience(all)

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