The hepatitis B virus posttranscriptional regulatory element is composed of two subelements

John E. Donello, Arlyne A. Beeche, George J. Smith, Ginger R. Lucero, Thomas J. Hope*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

80 Scopus citations

Abstract

The RNAs of the hepatitis B virus (HBV) contain a cis-acting regulatory element which facilitates the cytoplasmic localization of unspliced transcripts (J. Huang and T. J. Liang, Mol. Cell. Biol. 13:7476-7486, 1993, and Z. M. Huang and T. S. Yen, J. Virol. 68:3193-3199, 1994). Such localization is presumed to be mediated by cellular factors which interact with the element. The HBV posttranscriptional regulatory element (HBVPRE) can efficiently activate an RNA export reporter system in an orientation-dependent and position-independent manner. Deletion analysis reveals that the HBVPRE consists of two subelements which function synergistically. A synergistic effect was also observed when the 5′ (PREα) or 3′ (PREβ) subelements were duplicated. The bipartite structure of the HBVPRE is reminiscent of reports that the high-affinity binding sites of the Rev-like proteins must be duplicated to function efficiently (M. Grone, E. Hoffmann, S. Berchtold, B. R. Cullen, and R. Grassmann, Virology 204:144-152,1994; X. Huang, T. J. Hope, B. L. Bond, D. McDonald, K. Grahl, and T. G. Parslow, J. Virol. 65:2131-2134,1991; and D. McDonald, T. J. Hope, and T. G. Parslow, J. Virol. 66:7232-7238, 1992).

Original languageEnglish (US)
Pages (from-to)4345-4354
Number of pages10
JournalJournal of virology
Volume70
Issue number7
DOIs
StatePublished - 1996

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Fingerprint Dive into the research topics of 'The hepatitis B virus posttranscriptional regulatory element is composed of two subelements'. Together they form a unique fingerprint.

Cite this