The HIV-1 Vif PPLP motif is necessary for human APOBEC3G binding and degradation

John P. Donahue*, Michael L. Vetter, Nizar A. Mukhtar, Richard T. D'Aquila

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

54 Scopus citations

Abstract

The HIV-1 virion infectivity factor (Vif) is required during viral replication to inactivate the host cell anti-viral factor, APOBEC3G (A3G). Vif binds A3G and a Cullin5-ElonginBC E3 ubiquitin ligase complex which results in the proteasomal degradation of A3G. The Vif PPLP motif (amino acids 161-164) is essential for normal Vif function because mutations in this motif reduce the infectivity of virions produced in T-cells. In this report, we demonstrate that mutation of the Vif PPLP motif reduces Vif binding to A3G without affecting its interaction with ElonginC and Cullin5. We demonstrate that the failure of the Vif mutant to bind A3G resulted in A3G incorporation into assembling virions with loss of viral infectivity.

Original languageEnglish (US)
Pages (from-to)49-53
Number of pages5
JournalVirology
Volume377
Issue number1
DOIs
StatePublished - Jul 20 2008

Keywords

  • APOBEC3G
  • HIV-1 Vif
  • Vif PPLP motif
  • Vif multimerization

ASJC Scopus subject areas

  • Virology

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