The human FSGS-causing ANLN R431C mutation induces dysregulated PI3K/AKT/mTOR/Rac1 signaling in podocytes

Gentzon Hall, Brandon M. Lane, Kamal Khan, Igor Pediaditakis, Jianqiu Xiao, Guanghong Wu, Liming Wang, Maria E. Kovalik, Megan Chryst-Stangl, Erica Ellen Davis, Robert F. Spurney, Rasheed A. Gbadegesin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


Background We previously reported that mutations in the anillin (ANLN) gene cause familial forms of FSGS. ANLN is an F-actin binding protein that modulates podocyte cell motility and interacts with the phosphoinositide 3-kinase (PI3K) pathway through the slit diaphragm adaptor protein CD2-associated protein (CD2AP). However, it is unclear how the ANLN mutations cause the FSGS phenotype. We hypothesized that the R431C mutation exerts its pathogenic effects by uncoupling ANLN from CD2AP. Methods We conducted in vivo complementation assays in zebrafish to determine the effect of the previously identified missense ANLN variants, ANLN R431C and ANLN G618C during development. We also performed in vitro functional assays using human podocyte cell lines stably expressing wild-type ANLN (ANLN WT ) or ANLN R431C . Results Experiments in anln-deficient zebrafish embryos showed a loss-of-function effect for each ANLN variant. In human podocyte lines, expression of ANLN R431C increased cell migration, proliferation, and apoptosis. Biochemical characterization of ANLN R431C -expressing podocytes revealed hyperactivation of the PI3K/AKT/mTOR/p70S6K/Rac1 signaling axis and activation of mTOR-driven endoplasmic reticulum stress in ANLN R431C -expressing podocytes. Inhibition of mTOR, GSK-3b, Rac1, or calcineurin ameliorated the effects of ANLN R431C . Additionally, inhibition of the calcineurin/NFAT pathway reduced the expression of endogenous ANLN and mTOR. Conclusions The ANLN R431C mutation causes multiple derangements in podocyte function through hyperactivation of PI3K/AKT/mTOR/p70S6K/Rac1 signaling. Our findings suggest that the benefits of calcineurin inhibition in FSGS may be due, in part, to the suppression of ANLN and mTOR. Moreover, these studies illustrate that rational therapeutic targets for familial FSGS can be identified through biochemical characterization of dysregulated podocyte phenotypes.

Original languageEnglish (US)
Pages (from-to)2110-2122
Number of pages13
JournalJournal of the American Society of Nephrology
Issue number8
StatePublished - Aug 2018

ASJC Scopus subject areas

  • Nephrology


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