The human GCOM1 complex gene interacts with the NMDA receptor and internexin-alpha

Raymond S. Roginski*, Chi W. Lau, Phillip Pasquale Santoiemma, Sara J. Weaver, Peicheng Du, Patricia Soteropoulos, Jay Yang

*Corresponding author for this work

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The known functions of the human GCOM1 complex hub gene include transcription elongation and the intercalated disk of cardiac myocytes. However, in all likelihood, the gene's most interesting, and thus far least understood, roles will be found in the central nervous system. To investigate the functions of the GCOM1 gene in the CNS, we have cloned human and rat brain cDNAs encoding novel, 105 kDa GCOM1 combined (Gcom) proteins, designated Gcom15, and identified a new group of GCOM1 interacting genes, termed Gints, from yeast two-hybrid (Y2H) screens. We showed that Gcom15 interacts with the NR1 subunit of the NMDA receptor by co-expression in heterologous cells, in which we observed bi-directional co-immunoprecipitation of human Gcom15 and murine NR1. Our Y2H screens revealed 27 novel GCOM1 interacting genes, many of which are synaptic proteins and/or play roles in neurologic diseases. Finally, we showed, using rat brain protein preparations, that the Gint internexin-alpha (INA), a known interactor of the NMDAR, co-IPs with GCOM1 proteins, suggesting a GCOM1-GRIN1-INA interaction and a novel pathway that may be relevant to neuroprotection.

Original languageEnglish (US)
Pages (from-to)42-53
Number of pages12
JournalGene
Volume648
DOIs
StatePublished - Mar 30 2018

Fingerprint

N-Methyl-D-Aspartate Receptors
Genes
Proteins
Brain
Nervous System Diseases
Immunoprecipitation
Cardiac Myocytes
Central Nervous System
Complementary DNA
Yeasts
alpha-internexin

Keywords

  • GRIN1 gene
  • Gcom15 protein
  • Gdown1/POLR2M
  • Gup1/MYZAP
  • INA gene
  • Yeast two-hybrid

ASJC Scopus subject areas

  • Genetics

Cite this

Roginski, R. S., Lau, C. W., Santoiemma, P. P., Weaver, S. J., Du, P., Soteropoulos, P., & Yang, J. (2018). The human GCOM1 complex gene interacts with the NMDA receptor and internexin-alpha. Gene, 648, 42-53. https://doi.org/10.1016/j.gene.2018.01.029
Roginski, Raymond S. ; Lau, Chi W. ; Santoiemma, Phillip Pasquale ; Weaver, Sara J. ; Du, Peicheng ; Soteropoulos, Patricia ; Yang, Jay. / The human GCOM1 complex gene interacts with the NMDA receptor and internexin-alpha. In: Gene. 2018 ; Vol. 648. pp. 42-53.
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abstract = "The known functions of the human GCOM1 complex hub gene include transcription elongation and the intercalated disk of cardiac myocytes. However, in all likelihood, the gene's most interesting, and thus far least understood, roles will be found in the central nervous system. To investigate the functions of the GCOM1 gene in the CNS, we have cloned human and rat brain cDNAs encoding novel, 105 kDa GCOM1 combined (Gcom) proteins, designated Gcom15, and identified a new group of GCOM1 interacting genes, termed Gints, from yeast two-hybrid (Y2H) screens. We showed that Gcom15 interacts with the NR1 subunit of the NMDA receptor by co-expression in heterologous cells, in which we observed bi-directional co-immunoprecipitation of human Gcom15 and murine NR1. Our Y2H screens revealed 27 novel GCOM1 interacting genes, many of which are synaptic proteins and/or play roles in neurologic diseases. Finally, we showed, using rat brain protein preparations, that the Gint internexin-alpha (INA), a known interactor of the NMDAR, co-IPs with GCOM1 proteins, suggesting a GCOM1-GRIN1-INA interaction and a novel pathway that may be relevant to neuroprotection.",
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Roginski, RS, Lau, CW, Santoiemma, PP, Weaver, SJ, Du, P, Soteropoulos, P & Yang, J 2018, 'The human GCOM1 complex gene interacts with the NMDA receptor and internexin-alpha', Gene, vol. 648, pp. 42-53. https://doi.org/10.1016/j.gene.2018.01.029

The human GCOM1 complex gene interacts with the NMDA receptor and internexin-alpha. / Roginski, Raymond S.; Lau, Chi W.; Santoiemma, Phillip Pasquale; Weaver, Sara J.; Du, Peicheng; Soteropoulos, Patricia; Yang, Jay.

In: Gene, Vol. 648, 30.03.2018, p. 42-53.

Research output: Contribution to journalArticle

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T1 - The human GCOM1 complex gene interacts with the NMDA receptor and internexin-alpha

AU - Roginski, Raymond S.

AU - Lau, Chi W.

AU - Santoiemma, Phillip Pasquale

AU - Weaver, Sara J.

AU - Du, Peicheng

AU - Soteropoulos, Patricia

AU - Yang, Jay

PY - 2018/3/30

Y1 - 2018/3/30

N2 - The known functions of the human GCOM1 complex hub gene include transcription elongation and the intercalated disk of cardiac myocytes. However, in all likelihood, the gene's most interesting, and thus far least understood, roles will be found in the central nervous system. To investigate the functions of the GCOM1 gene in the CNS, we have cloned human and rat brain cDNAs encoding novel, 105 kDa GCOM1 combined (Gcom) proteins, designated Gcom15, and identified a new group of GCOM1 interacting genes, termed Gints, from yeast two-hybrid (Y2H) screens. We showed that Gcom15 interacts with the NR1 subunit of the NMDA receptor by co-expression in heterologous cells, in which we observed bi-directional co-immunoprecipitation of human Gcom15 and murine NR1. Our Y2H screens revealed 27 novel GCOM1 interacting genes, many of which are synaptic proteins and/or play roles in neurologic diseases. Finally, we showed, using rat brain protein preparations, that the Gint internexin-alpha (INA), a known interactor of the NMDAR, co-IPs with GCOM1 proteins, suggesting a GCOM1-GRIN1-INA interaction and a novel pathway that may be relevant to neuroprotection.

AB - The known functions of the human GCOM1 complex hub gene include transcription elongation and the intercalated disk of cardiac myocytes. However, in all likelihood, the gene's most interesting, and thus far least understood, roles will be found in the central nervous system. To investigate the functions of the GCOM1 gene in the CNS, we have cloned human and rat brain cDNAs encoding novel, 105 kDa GCOM1 combined (Gcom) proteins, designated Gcom15, and identified a new group of GCOM1 interacting genes, termed Gints, from yeast two-hybrid (Y2H) screens. We showed that Gcom15 interacts with the NR1 subunit of the NMDA receptor by co-expression in heterologous cells, in which we observed bi-directional co-immunoprecipitation of human Gcom15 and murine NR1. Our Y2H screens revealed 27 novel GCOM1 interacting genes, many of which are synaptic proteins and/or play roles in neurologic diseases. Finally, we showed, using rat brain protein preparations, that the Gint internexin-alpha (INA), a known interactor of the NMDAR, co-IPs with GCOM1 proteins, suggesting a GCOM1-GRIN1-INA interaction and a novel pathway that may be relevant to neuroprotection.

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KW - INA gene

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Roginski RS, Lau CW, Santoiemma PP, Weaver SJ, Du P, Soteropoulos P et al. The human GCOM1 complex gene interacts with the NMDA receptor and internexin-alpha. Gene. 2018 Mar 30;648:42-53. https://doi.org/10.1016/j.gene.2018.01.029