The hydraulic conductivity of Matrigel™

William J. Mccarty, Mark Johnson*

*Corresponding author for this work

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

In this study, we measured the specific hydraulic conductivity (K) of Matrigel™ at 1% and 2% concentrations as a function of perfusion pressure (0 to 100 mmHg) and compared the results to predictions from two models: a fiber matrix model that predicted K of the gel based upon its composition, and a biphasic model that predicted changes in K caused by pressure induced compaction of the gels. The extent of gel compaction as a function of perfusion pressure was also assessed, allowing us to estimate the stiffness of the gels. As expected, 2% Matrigel™ had a lower K and a higher stiffness than did 1% Matrigel™. Measured values of K of both 1% and 2% Matrigel™ samples showed good agreement with the predictions of the fiber matrix model. Pressure-induced changes in K were better described by the biphasic model than a model in which uniform compression of the gel was assumed. We conclude that K of multi-component gels, such as Matrigel™can be well characterized by fiber matrix models, and that pressure-induced changes in K of such gels can be well characterized by biphasic models.

Original languageEnglish (US)
Pages (from-to)303-317
Number of pages15
JournalBiorheology
Volume44
Issue number5-6
StatePublished - Dec 1 2007

Keywords

  • Basement membrane
  • Biphasic
  • Fiber matrix model

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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    Mccarty, W. J., & Johnson, M. (2007). The hydraulic conductivity of Matrigel™. Biorheology, 44(5-6), 303-317.