Abstract
We have used a tumorigenic glioblastoma cell line, SNB-19, as a model system to identify fucose-containing glycoprotein candidates for tumor suppressor function. Glycoproteins were analyzed after treatment with a variety of chemical differentiating agents by two-dimensional SDS-PAGE, followed by electroblotting and visualization using the fucose-specific lectin, Ulex europeaus I. Approximately 25 fucose-containing glycoproteins (FUCGLAPs) were routinely visualized in control extracts using 60-70 μg of protein per gel and staining with Vectastain ABC kits. Retinoic acid induced the most marked change in FUCGLAP expression, causing a fivelold increase in one FUCGLAP (M r =125 kDa, pI=6.6) Neither butyric acid, dibutyryl cAMP, nor combinations of these compounds gave a similar result. Using this model system and analytical approach, it should be possible to identify, isolate, and evaluate glycoprotein oligosaccharides for their tumor modulating capability.
Original language | English (US) |
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Pages (from-to) | 311-327 |
Number of pages | 17 |
Journal | Molecular and Chemical Neuropathology |
Volume | 21 |
Issue number | 2-3 |
DOIs | |
State | Published - Feb 1994 |
Funding
Keywords
- Fucoproteins
- butyric acid
- cAMP
- cell-surface glycoconjugates
- glioblastoma
- glycoproteins
- oligosaccharides
- retinoic acid
ASJC Scopus subject areas
- Clinical Neurology
- Molecular Biology
- General Neuroscience