The identification of glioblastoma-associatied, fucose-containing glycoproteins induced by retinoic acid

David L. VanderMeulen, V. V T S Prasad, Joseph R. Moskal*

*Corresponding author for this work

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

We have used a tumorigenic glioblastoma cell line, SNB-19, as a model system to identify fucose-containing glycoprotein candidates for tumor suppressor function. Glycoproteins were analyzed after treatment with a variety of chemical differentiating agents by two-dimensional SDS-PAGE, followed by electroblotting and visualization using the fucose-specific lectin, Ulex europeaus I. Approximately 25 fucose-containing glycoproteins (FUCGLAPs) were routinely visualized in control extracts using 60-70 μg of protein per gel and staining with Vectastain ABC kits. Retinoic acid induced the most marked change in FUCGLAP expression, causing a fivelold increase in one FUCGLAP (M r =125 kDa, pI=6.6) Neither butyric acid, dibutyryl cAMP, nor combinations of these compounds gave a similar result. Using this model system and analytical approach, it should be possible to identify, isolate, and evaluate glycoprotein oligosaccharides for their tumor modulating capability.

Original languageEnglish (US)
Pages (from-to)311-327
Number of pages17
JournalMolecular and Chemical Neuropathology
Volume21
Issue number2-3
DOIs
StatePublished - Feb 1 1994

Keywords

  • Fucoproteins
  • butyric acid
  • cAMP
  • cell-surface glycoconjugates
  • glioblastoma
  • glycoproteins
  • oligosaccharides
  • retinoic acid

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology

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