The impact of temperature and pump flow rate during selective cerebral perfusion on regional blood flow in piglets

Jian Wang, Richard M. Ginther, Matthew Riegel, Rong Huang, Mahesh S. Sharma, Kristine J. Guleserian, Joseph M. Forbess*

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objective: Ideal temperature and flow rate for selective cerebral perfusion (SCP) are not known. We examined regional organ perfusion in a piglet SCP model. Methods: Three groups underwent SCP at 30 mL/kg/min at different temperatures (15°C, 25°C, and 32°C) and 4 groups remained at 25°C for SCP at different flow rates (10, 30, 50 and 75 mL/kg/min). Fluorescent microspheres were injected at 5 minutes of normothermic cardiopulmonary bypass (CPB), immediately before SCP, SCP 45 minutes, SCP 90 minutes, and 2 hours after CPB. Brain and lower body organs were collected to examine regional blood flow (RBF, mL/min/g). Results: At 2 hours after CPB, RBF of the 32°C group was higher than that of the 15°C group (P < .05) at the caudate nucleus and hippocampus; RBF of the 32°C group was higher than that of the 25°C and 15°C groups (P < .05) at the neocortex. No significant difference in RBF was observed among any of the 25°C groups at different flow rates. Also, there was no significant difference between the RBF to the left and right sides of brain in either the temperature or flow rate groups. RBF did significantly increase with temperature in the liver and quadriceps during SCP (P < .05). At the kidney, RBF at SCP 90 minutes was significantly higher than that at SCP 45 minutes when all temperature groups were combined (P < .05). Conclusions: SCP at 32°C provides higher brain RBF 2 hours after CPB. Increasing SCP flow rate does not increase RBF significantly at 25°C. Higher temperature during SCP results in improved RBF to the liver and quadriceps.

Original languageEnglish (US)
Pages (from-to)188-195
Number of pages8
JournalJournal of Thoracic and Cardiovascular Surgery
Volume145
Issue number1
DOIs
StatePublished - Jan 1 2013

Fingerprint

Regional Blood Flow
Perfusion
Temperature
Cardiopulmonary Bypass
Brain
Caudate Nucleus
Liver
Neocortex
Microspheres
Hippocampus

ASJC Scopus subject areas

  • Surgery
  • Pulmonary and Respiratory Medicine
  • Cardiology and Cardiovascular Medicine

Cite this

Wang, Jian ; Ginther, Richard M. ; Riegel, Matthew ; Huang, Rong ; Sharma, Mahesh S. ; Guleserian, Kristine J. ; Forbess, Joseph M. / The impact of temperature and pump flow rate during selective cerebral perfusion on regional blood flow in piglets. In: Journal of Thoracic and Cardiovascular Surgery. 2013 ; Vol. 145, No. 1. pp. 188-195.
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abstract = "Objective: Ideal temperature and flow rate for selective cerebral perfusion (SCP) are not known. We examined regional organ perfusion in a piglet SCP model. Methods: Three groups underwent SCP at 30 mL/kg/min at different temperatures (15°C, 25°C, and 32°C) and 4 groups remained at 25°C for SCP at different flow rates (10, 30, 50 and 75 mL/kg/min). Fluorescent microspheres were injected at 5 minutes of normothermic cardiopulmonary bypass (CPB), immediately before SCP, SCP 45 minutes, SCP 90 minutes, and 2 hours after CPB. Brain and lower body organs were collected to examine regional blood flow (RBF, mL/min/g). Results: At 2 hours after CPB, RBF of the 32°C group was higher than that of the 15°C group (P < .05) at the caudate nucleus and hippocampus; RBF of the 32°C group was higher than that of the 25°C and 15°C groups (P < .05) at the neocortex. No significant difference in RBF was observed among any of the 25°C groups at different flow rates. Also, there was no significant difference between the RBF to the left and right sides of brain in either the temperature or flow rate groups. RBF did significantly increase with temperature in the liver and quadriceps during SCP (P < .05). At the kidney, RBF at SCP 90 minutes was significantly higher than that at SCP 45 minutes when all temperature groups were combined (P < .05). Conclusions: SCP at 32°C provides higher brain RBF 2 hours after CPB. Increasing SCP flow rate does not increase RBF significantly at 25°C. Higher temperature during SCP results in improved RBF to the liver and quadriceps.",
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The impact of temperature and pump flow rate during selective cerebral perfusion on regional blood flow in piglets. / Wang, Jian; Ginther, Richard M.; Riegel, Matthew; Huang, Rong; Sharma, Mahesh S.; Guleserian, Kristine J.; Forbess, Joseph M.

In: Journal of Thoracic and Cardiovascular Surgery, Vol. 145, No. 1, 01.01.2013, p. 188-195.

Research output: Contribution to journalArticle

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T1 - The impact of temperature and pump flow rate during selective cerebral perfusion on regional blood flow in piglets

AU - Wang, Jian

AU - Ginther, Richard M.

AU - Riegel, Matthew

AU - Huang, Rong

AU - Sharma, Mahesh S.

AU - Guleserian, Kristine J.

AU - Forbess, Joseph M.

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N2 - Objective: Ideal temperature and flow rate for selective cerebral perfusion (SCP) are not known. We examined regional organ perfusion in a piglet SCP model. Methods: Three groups underwent SCP at 30 mL/kg/min at different temperatures (15°C, 25°C, and 32°C) and 4 groups remained at 25°C for SCP at different flow rates (10, 30, 50 and 75 mL/kg/min). Fluorescent microspheres were injected at 5 minutes of normothermic cardiopulmonary bypass (CPB), immediately before SCP, SCP 45 minutes, SCP 90 minutes, and 2 hours after CPB. Brain and lower body organs were collected to examine regional blood flow (RBF, mL/min/g). Results: At 2 hours after CPB, RBF of the 32°C group was higher than that of the 15°C group (P < .05) at the caudate nucleus and hippocampus; RBF of the 32°C group was higher than that of the 25°C and 15°C groups (P < .05) at the neocortex. No significant difference in RBF was observed among any of the 25°C groups at different flow rates. Also, there was no significant difference between the RBF to the left and right sides of brain in either the temperature or flow rate groups. RBF did significantly increase with temperature in the liver and quadriceps during SCP (P < .05). At the kidney, RBF at SCP 90 minutes was significantly higher than that at SCP 45 minutes when all temperature groups were combined (P < .05). Conclusions: SCP at 32°C provides higher brain RBF 2 hours after CPB. Increasing SCP flow rate does not increase RBF significantly at 25°C. Higher temperature during SCP results in improved RBF to the liver and quadriceps.

AB - Objective: Ideal temperature and flow rate for selective cerebral perfusion (SCP) are not known. We examined regional organ perfusion in a piglet SCP model. Methods: Three groups underwent SCP at 30 mL/kg/min at different temperatures (15°C, 25°C, and 32°C) and 4 groups remained at 25°C for SCP at different flow rates (10, 30, 50 and 75 mL/kg/min). Fluorescent microspheres were injected at 5 minutes of normothermic cardiopulmonary bypass (CPB), immediately before SCP, SCP 45 minutes, SCP 90 minutes, and 2 hours after CPB. Brain and lower body organs were collected to examine regional blood flow (RBF, mL/min/g). Results: At 2 hours after CPB, RBF of the 32°C group was higher than that of the 15°C group (P < .05) at the caudate nucleus and hippocampus; RBF of the 32°C group was higher than that of the 25°C and 15°C groups (P < .05) at the neocortex. No significant difference in RBF was observed among any of the 25°C groups at different flow rates. Also, there was no significant difference between the RBF to the left and right sides of brain in either the temperature or flow rate groups. RBF did significantly increase with temperature in the liver and quadriceps during SCP (P < .05). At the kidney, RBF at SCP 90 minutes was significantly higher than that at SCP 45 minutes when all temperature groups were combined (P < .05). Conclusions: SCP at 32°C provides higher brain RBF 2 hours after CPB. Increasing SCP flow rate does not increase RBF significantly at 25°C. Higher temperature during SCP results in improved RBF to the liver and quadriceps.

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