The importance of purity in the crystallization of DNA binding immunoglobulin Fab fragments

Wayne F. Anderson*, Amechand Boodhoo, Clifford D. Mol

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

As part of a program to determine the structures of a number of immunoglobulin Fab fragments that are specific for different DNA structures or sequences, the affects of sample purity upon crystallization have been studied. The protease treatment utilized to produce Fab fragments introduces micro-heterogeneity into the sample which is detrimental to crystal growth. Purification of a single Fab species was found to be an essential step in obtaining large single crystals. If crystallization experiments on biological macromolecules yield only microcrystals or if crystallization is not reproducible the most likely causes for these problems are insufficient purity of the sample and variation in the level of purity.

Original languageEnglish (US)
Pages (from-to)153-159
Number of pages7
JournalJournal of Crystal Growth
Volume90
Issue number1-3
DOIs
StatePublished - Jul 2 1988

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Inorganic Chemistry
  • Materials Chemistry

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