The incorporation of intrastriatally injected [3H]fucose into electrophoretically separated synaptosomal glycoproteins. II. The influence of passive avoidance training

David G. Morgan*, Aryeh Routtenberg

*Corresponding author for this work

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Following intrastriatal injections of [3H]l-fucose, male albino rats served as (a) trained subjects in a step-down passive avoidance task, (b) stress controls receiving inescapable shock or (c) handled controls. At a series of time points after treatment the animals were sacrified and the P2 fraction of the injected neostriatum was isolated. This tissue was electrophoresed on SDS-polyacrylamide gels and radioactivity profiles were constructed from 1 mm gel slices. The profiles of trained subjects were compared to shocked and handled control subjects from the same time point group. No differences in total [3H]fucose incorporation into neostriatal glycoproteins were detected as a result of the behavioral treatment used, nor was an incorporation into the majority of electrophoresed peaks altered. Three radioactive gel peaks were significantly altered as a function of experience. At the one day time point, trained subjects exhibited a significant increase in the tritium content of a 70,000 dalton fucosylglycoprotein peak. At the 5 day time point, increased label was detected in a 180,000 dalton peak in both trained and shocked subjects, while a significant increase in a 140,000 dalton peak was observed only in trained animals. The relation of the present findings to previously reported training related differences in glycoprotein metabolism are discussed.

Original languageEnglish (US)
Pages (from-to)343-354
Number of pages12
JournalBrain research
Volume179
Issue number2
DOIs
StatePublished - Dec 28 1979

Keywords

  • [H]fucose
  • electrophoresis
  • glycoproteins
  • memory
  • neostriatum
  • passive avoidance
  • synapse

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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