Abstract
Following intrastriatal injections of [3H]l-fucose, male albino rats served as (a) trained subjects in a step-down passive avoidance task, (b) stress controls receiving inescapable shock or (c) handled controls. At a series of time points after treatment the animals were sacrified and the P2 fraction of the injected neostriatum was isolated. This tissue was electrophoresed on SDS-polyacrylamide gels and radioactivity profiles were constructed from 1 mm gel slices. The profiles of trained subjects were compared to shocked and handled control subjects from the same time point group. No differences in total [3H]fucose incorporation into neostriatal glycoproteins were detected as a result of the behavioral treatment used, nor was an incorporation into the majority of electrophoresed peaks altered. Three radioactive gel peaks were significantly altered as a function of experience. At the one day time point, trained subjects exhibited a significant increase in the tritium content of a 70,000 dalton fucosylglycoprotein peak. At the 5 day time point, increased label was detected in a 180,000 dalton peak in both trained and shocked subjects, while a significant increase in a 140,000 dalton peak was observed only in trained animals. The relation of the present findings to previously reported training related differences in glycoprotein metabolism are discussed.
Original language | English (US) |
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Pages (from-to) | 343-354 |
Number of pages | 12 |
Journal | Brain research |
Volume | 179 |
Issue number | 2 |
DOIs | |
State | Published - Dec 28 1979 |
Funding
We would like to thank Jim Drennan and Jesus Basadre for their expert technical assistance and Betty Wells for preparation of the manuscript. Supported in part by MH 25281 and NSF 19388 to A.R. Computer funds supplied by Northwestern University to D.M.
Keywords
- [H]fucose
- electrophoresis
- glycoproteins
- memory
- neostriatum
- passive avoidance
- synapse
ASJC Scopus subject areas
- General Neuroscience
- Molecular Biology
- Clinical Neurology
- Developmental Biology