Abstract
Following intrastriatal injections of [3H]l-fucose, male albino rats served as (a) trained subjects in a step-down passive avoidance task, (b) stress controls receiving inescapable shock or (c) handled controls. At a series of time points after treatment the animals were sacrified and the P2 fraction of the injected neostriatum was isolated. This tissue was electrophoresed on SDS-polyacrylamide gels and radioactivity profiles were constructed from 1 mm gel slices. The profiles of trained subjects were compared to shocked and handled control subjects from the same time point group. No differences in total [3H]fucose incorporation into neostriatal glycoproteins were detected as a result of the behavioral treatment used, nor was an incorporation into the majority of electrophoresed peaks altered. Three radioactive gel peaks were significantly altered as a function of experience. At the one day time point, trained subjects exhibited a significant increase in the tritium content of a 70,000 dalton fucosylglycoprotein peak. At the 5 day time point, increased label was detected in a 180,000 dalton peak in both trained and shocked subjects, while a significant increase in a 140,000 dalton peak was observed only in trained animals. The relation of the present findings to previously reported training related differences in glycoprotein metabolism are discussed.
Original language | English (US) |
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Pages (from-to) | 343-354 |
Number of pages | 12 |
Journal | Brain research |
Volume | 179 |
Issue number | 2 |
DOIs | |
State | Published - Dec 28 1979 |
Keywords
- [H]fucose
- electrophoresis
- glycoproteins
- memory
- neostriatum
- passive avoidance
- synapse
ASJC Scopus subject areas
- Neuroscience(all)
- Molecular Biology
- Clinical Neurology
- Developmental Biology