The Increase in Maternal Expression of axin1 and axin2 Contribute to the Zebrafish Mutant Ichabod Ventralized Phenotype

Fabio Valenti, Jessica Ibetti, Yuko Komiya, Melissa Baxter, Anna Maria Lucchese, Lauren Derstine, Claudia Covaciu, Valeria Rizzo, Renza Vento, Giuseppe Russo, Marcella Macaluso, Franco Cotelli, Daniele Castiglia, Cara J. Gottardi, Raymond Habas, Antonio Giordano, Gianfranco Bellipanni*

*Corresponding author for this work

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

β-catenin is a central effector of the Wnt pathway and one of the players in Ca+-dependent cell-cell adhesion. While many wnts are present and expressed in vertebrates, only one β-catenin exists in the majority of the organisms. One intriguing exception is zebrafish that carries two genes for β-catenin. The maternal recessive mutation ichabod presents very low levels of β-catenin2 that in turn affects dorsal axis formation, suggesting that β-catenin1 is incapable to compensate for β-catenin2 loss and raising the question of whether these two β-catenins may have differential roles during early axis specification. Here we identify a specific antibody that can discriminate selectively for β-catenin1. By confocal co-immunofluorescent analysis and low concentration gain-of-function experiments, we show that β-catenin1 and 2 behave in similar modes in dorsal axis induction and cellular localization. Surprisingly, we also found that in the ich embryo the mRNAs of the components of β-catenin regulatory pathway, including β-catenin1, are more abundant than in the Wt embryo. Increased levels of β-catenin1 are found at the membrane level but not in the nuclei till high stage. Finally, we present evidence that β-catenin1 cannot revert the ich phenotype because it may be under the control of a GSK3β-independent mechanism that required Axin's RGS domain function. J. Cell. Biochem. 116: 418-430, 2015.

Original languageEnglish (US)
Pages (from-to)418-430
Number of pages13
JournalJournal of Cellular Biochemistry
Volume116
Issue number3
DOIs
StatePublished - Mar 1 2015

Keywords

  • AXIS FORMATION
  • Axin2-RGS DOMAIN
  • BETA-CATENIN
  • Wnt signaling
  • ZEBRAFISH

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Valenti, F., Ibetti, J., Komiya, Y., Baxter, M., Lucchese, A. M., Derstine, L., Covaciu, C., Rizzo, V., Vento, R., Russo, G., Macaluso, M., Cotelli, F., Castiglia, D., Gottardi, C. J., Habas, R., Giordano, A., & Bellipanni, G. (2015). The Increase in Maternal Expression of axin1 and axin2 Contribute to the Zebrafish Mutant Ichabod Ventralized Phenotype. Journal of Cellular Biochemistry, 116(3), 418-430. https://doi.org/10.1002/jcb.24993