The induction of accelerated murine amyloid with human splenic extract - Probable role of amyloid enhancing factor

John Varga, Margaret S M Flinn, Tsuranobu Shirahama*, Orville G. Rodgers, Alan S. Cohen

*Corresponding author for this work

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

Amyloid enhancing factor (AEF) is derived from the tissues of pre-amyloidotic and amyloidotic animals and, when transfered, greatly accelerates amyloid induction in the recipient murine models. It has also been reported that similarly accelerated amyloid induction can be achieved in mice by injection of human splenic homogenates from patients with amyloidosis. The present study has attempted to characterize further the mechanism of this "heterologous transfer of amyloid". Treatment of mice with the "tissue homogenate" or the "AEF extract" of AA-, AL-and Aprealbumin-laden human spleens followed by daily subcutaneous casein injections induced amyloidosis in an accelerated fashion. The resultant amyloid deposits in mice had strongly positive immunohistochemical reactions with anti-mouse AA, and negative reaction with anti-human AA or anti-human prealbumin. The results lend support to the idea that accelerated amyloid induction in the recipient mice is unlikely to be due to transfer of human amyloid substance, but rather to formation of "native" murine amyloid under the influence of a human AEF factor similar to or identical with AEF described in mouse-to mouse transfer models.

Original languageEnglish (US)
Pages (from-to)177-185
Number of pages9
JournalVirchows Archiv B Cell Pathology Including Molecular Pathology
Volume51
Issue number1
DOIs
StatePublished - Dec 1 1986

Keywords

  • Amyloid
  • Amyloid enhancing factor
  • Cross-species compatibility

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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