The Inflammatory Potential of Dietary Manganese in a Cohort of Elderly Men

Jacob K. Kresovich*, Catherine M. Bulka, Brian T. Joyce, Pantel S. Vokonas, Joel Schwartz, Andrea A. Baccarelli, Elizabeth A. Hibler, Lifang Hou

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


Manganese is an essential nutrient that may play a role in the production of inflammatory biomarkers. We examined associations between estimated dietary manganese intake from food/beverages and supplements with circulating biomarkers of inflammation. We further explored whether estimated dietary manganese intake affects DNA methylation of selected genes involved in the production of these biomarkers. We analyzed 1023 repeated measures of estimated dietary manganese intakes and circulating blood inflammatory biomarkers from 633 participants in the Normative Aging Study. Using mixed-effect linear regression models adjusted for covariates, we observed positive linear trends between estimated dietary manganese intakes and three circulating interleukin proteins. Relative to the lowest quartile of estimated intake, concentrations of IL-1β were 46% greater (95% CI − 5, 126), IL-6 52% greater (95% CI − 9, 156). and IL-8 32% greater (95% CI 2, 71) in the highest quartiles of estimated intake. Estimated dietary manganese intake was additionally associated with changes in DNA methylation of inflammatory biomarker-producing genes. Higher estimated intake was associated with higher methylation of NF-κβ member activator NKAP (Q4 vs Q1: β = 3.32, 95% CI − 0.6, 7.3). When stratified by regulatory function, higher manganese intake was associated with higher gene body methylation of NF-κβ member activators NKAP (Q4 vs Q1: β = 10.10, 95% CI − 0.8, 21) and NKAPP1 (Q4 vs Q1: β = 8.14, 95% CI 1.1, 15). While needed at trace amounts for various physiologic functions, our results suggest estimated dietary intakes of manganese at levels slightly above nutritional adequacy contribute to inflammatory biomarker production.

Original languageEnglish (US)
Pages (from-to)49-57
Number of pages9
JournalBiological Trace Element Research
Issue number1
StatePublished - May 1 2018


  • Cytokines
  • DNA methylation
  • Dietary manganese
  • Inflammation
  • Manganese

ASJC Scopus subject areas

  • Biochemistry, medical
  • Biochemistry
  • Clinical Biochemistry
  • Inorganic Chemistry
  • Endocrinology, Diabetes and Metabolism


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