TY - JOUR
T1 - The influence of carbon-encapsulated iron nanoparticles on elastic modulus of living human mesenchymal stem cells examined by atomic force microscopy
AU - Dulinska-Molak, Ida
AU - Chlanda, Adrian
AU - Li, Jasmine
AU - Wang, Xinlong
AU - Bystrzejewski, Michal
AU - Kawazoe, Naoki
AU - Chen, Guoping
AU - Swieszkowski, Wojciech
N1 - Funding Information:
This work was supported by the Faculty of Materials Science and Engineering WUT , grant numer 504/02185/1090/42.000000 .
Publisher Copyright:
© 2018 Elsevier Ltd
PY - 2018/5
Y1 - 2018/5
N2 - Nanomaterials and nanoparticles are regarded as promising candidates for various biomedical applications due to their unique physicochemical properties. In this study, three types of carbon-encapsulated iron nanoparticles (CEINs) were synthesized and their impact on cellular changes was investigated by atomic force microscopy (AFM). The AFM experiment was additionally compared with conventional methods, such as colorimetric assay and other microscopic techniques. A significant difference of reduced Young's modulus of the cells was revealed, even at low concentration of nanoparticles in the culture medium. The AFM measurement proved to be a useful tool not only for visualization, but also for identification of local cellular changes at the nanoscale after exposure to carbon-encapsulated iron nanoparticles.
AB - Nanomaterials and nanoparticles are regarded as promising candidates for various biomedical applications due to their unique physicochemical properties. In this study, three types of carbon-encapsulated iron nanoparticles (CEINs) were synthesized and their impact on cellular changes was investigated by atomic force microscopy (AFM). The AFM experiment was additionally compared with conventional methods, such as colorimetric assay and other microscopic techniques. A significant difference of reduced Young's modulus of the cells was revealed, even at low concentration of nanoparticles in the culture medium. The AFM measurement proved to be a useful tool not only for visualization, but also for identification of local cellular changes at the nanoscale after exposure to carbon-encapsulated iron nanoparticles.
KW - Atomic force microscopy
KW - Carbon-encapsulated iron nanoparticles
KW - Force spectroscopy
KW - Nanomaterial
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U2 - 10.1016/j.micron.2018.02.006
DO - 10.1016/j.micron.2018.02.006
M3 - Article
C2 - 29574392
AN - SCOPUS:85044125768
SN - 0968-4328
VL - 108
SP - 41
EP - 48
JO - Micron
JF - Micron
ER -