The influence of fibrin on the reaction of plasminogen activation by mutant of pro-urokinase

Xin Dang, Jing Xin Yang, Qiang Ru, Hong Sheng Yuan, Bing Gen Ru*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Because the influence of fibrin on the reaction of plasminogen activation by various plasminogen activators is different, the kinetic constant of the reaction of plasminogen activation catalyzed by InB with and without fibrin were detected. The result is: Kmfibrin = 4.2 μmol · L-1, greater than the normal Km = 0.379 μmol·L-1; kcatfibrin = 0.107 s-1, greater than the normal kcat = 0.0165 s-1. The results suggest that existence of fibrin in the reaction system of plasminogen activation depress the affinity between InB and plasminogen, but accelerates the hydrolysis of plasminogen by InB. The count up effect is inhibition.

Original languageEnglish (US)
Pages (from-to)212-213
Number of pages2
JournalProgress in Biochemistry and Biophysics
Volume28
Issue number2
StatePublished - Dec 1 2001
Externally publishedYes

Keywords

  • Fibrin
  • Kinetics
  • Mutant of single-chain urokinase-type plasminogen activator
  • Plasminogen

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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