The innate immune factor apolipoprotein L1 restricts HIV-1 infection

Harry E. Taylor, Atanu K. Khatua, Waldemar Popik*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

82 Scopus citations


Apolipoprotein L1 (APOL1) is a major component of the human innate immune response against African trypanosomes. Although the mechanism of the trypanolytic activity of circulating APOL1 has been recently clarified, the intracellular function(s) of APOL1 in human cells remains poorly defined. Like that of many genes linked to host immunity, APOL1 expression is induced by proinflammatory cytokines gamma interferon (IFN-α) and tumor necrosis factor alpha (TNF-γ). Additionally, IFN-α-polarized macrophages that potently restrict HIV-1 replication express APOL1, which suggests that APOL1 may contribute to HIV-1 suppression. Here, we report that APOL1 inhibits HIV-1 replication by multiple mechanisms. We found that APOL1 protein targeted HIV-1 Gag for degradation by the endolysosomal pathway. Interestingly, we found that APOL1 stimulated both endocytosis and lysosomal biogenesis by promoting nuclear localization of transcription factor EB (TFEB) and expression of TFEB target genes. Moreover, we demonstrated that APOL1 depletes cellular viral accessory protein Vif, which counteracts the host restriction factor APOBEC3G, via a pathway involving degradation of Vif in lysosomes and by secretion of Vif in microvesicles. As a result of Vif depletion by APOL1, APOBEC3G was not degraded and reduced infectivity of progeny virions. In support of this model, we also showed that endogenous expression of APOL1 in differentiated U937 monocytic cells stimulated with IFN-α resulted in a reduced production of virus particles. This finding supports the hypothesis that induction of APOL1 contributes to HIV-1 suppression in differentiated monocytes. Deciphering the precise mechanism of APOL1-mediated HIV-1 restriction may facilitate the design of unique therapeutics to target HIV-1 replication.

Original languageEnglish (US)
Pages (from-to)592-603
Number of pages12
JournalJournal of virology
Issue number1
StatePublished - Jan 2014

ASJC Scopus subject areas

  • Insect Science
  • Virology
  • Microbiology
  • Immunology


Dive into the research topics of 'The innate immune factor apolipoprotein L1 restricts HIV-1 infection'. Together they form a unique fingerprint.

Cite this